Document Detail


Germline KRAS mutations cause Noonan syndrome.
MedLine Citation:
PMID:  16474405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Noonan syndrome (MIM 163950) is characterized by short stature, facial dysmorphism and cardiac defects. Heterozygous mutations in PTPN11, which encodes SHP-2, cause approximately 50% of cases of Noonan syndrome. The SHP-2 phosphatase relays signals from activated receptor complexes to downstream effectors, including Ras. We discovered de novo germline KRAS mutations that introduce V14I, T58I or D153V amino acid substitutions in five individuals with Noonan syndrome and a P34R alteration in a individual with cardio-facio-cutaneous syndrome (MIM 115150), which has overlapping features with Noonan syndrome. Recombinant V14I and T58I K-Ras proteins show defective intrinsic GTP hydrolysis and impaired responsiveness to GTPase activating proteins, render primary hematopoietic progenitors hypersensitive to growth factors and deregulate signal transduction in a cell lineage-specific manner. These studies establish germline KRAS mutations as a cause of human disease and infer that the constellation of developmental abnormalities seen in Noonan syndrome spectrum is, in large part, due to hyperactive Ras.
Authors:
Suzanne Schubbert; Martin Zenker; Sara L Rowe; Silke Böll; Cornelia Klein; Gideon Bollag; Ineke van der Burgt; Luciana Musante; Vera Kalscheuer; Lars-Erik Wehner; Hoa Nguyen; Brian West; Kam Y J Zhang; Erik Sistermans; Anita Rauch; Charlotte M Niemeyer; Kevin Shannon; Christian P Kratz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-02-12
Journal Detail:
Title:  Nature genetics     Volume:  38     ISSN:  1061-4036     ISO Abbreviation:  Nat. Genet.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-27     Completed Date:  2006-05-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9216904     Medline TA:  Nat Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  331-6     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of California, 513 Parnassus Avenue, San Francisco, California 94143, USA.
Data Bank Information
Bank Name/Acc. No.:
OMIM/115150;  163950
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Female
Genes, ras*
Germ-Line Mutation*
Guanosine Triphosphate / metabolism
Heterozygote Detection
Humans
Infant
Intracellular Signaling Peptides and Proteins / genetics
Male
Noonan Syndrome / genetics*
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatases / genetics
Grant Support
ID/Acronym/Agency:
R01 CA104282/CA/NCI NIH HHS; R01 CA72614/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Intracellular Signaling Peptides and Proteins; 86-01-1/Guanosine Triphosphate; EC 3.1.3.48/PTPN11 protein, human; EC 3.1.3.48/Protein Tyrosine Phosphatase, Non-Receptor Type 11; EC 3.1.3.48/Protein Tyrosine Phosphatases
Comments/Corrections
Erratum In:
Nat Genet. 2006 May;38(5):598

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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