| Germination Dramatically Increases Isoflavonoid Content and Diversity in Chickpea (Cicer arietinum L.) Seeds. | |
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MedLine Citation:
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PMID: 22816801 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ABSTRACT The effect of germination on bioactive components in legume seeds were investigated in terms of the antioxidant capacity and total phenolic contents. Germination increased total phenolic content and antioxidant capacity of most seeds. Particularly in chickpea seeds, the isoflavone contents increased by over 100 fold, mainly due to increase of formononetin and biochanin A level. As a result, these two compounds were conveniently isolated from the germinated seeds in preparative scale and structurally confirmed by UV/vis, ESI-MS, and 1H NMR spectroscopy. Isoflavonoids fingerprints analyzed by HPLC-PDA and LC-ESI-MS demonstrated that germination could significantly increase isoflavonoids diversity. Twenty-five isoflavonoids were detected and identified tentatively. These include 20 isoflavones, 2 isoflavanones and 3 pterocarpan phytoalexins. Total isoflavonoid content of germinated chickpea was approximately 5-fold of that of germinated soybean. Our findings suggested that the germinated chickpea seeds could serve as a promising functional food rich in isoflavonoids. |
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Authors:
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Ziyun Wu; Lixia Song; Shengbao Feng; Yuancai Liu; Guangyuan He; Yoecelyn Yioe; Shao Quan Liu; Dejian Huang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-23 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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