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Germinal cell mutagenesis in specially designed maize genotypes.
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MedLine Citation:
PMID:  6780335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used three inbreds of Zea mays in our in situ and laboratory studies in environmental mutagenesis. Inbred W22 plants homozygous for wx-C were used in a study to detect the possible mutagenic properties of 32 pesticides or combination of pesticides under modern agricultural conditions. The large numbers of pollen grains analyzed and the ease in detecting mutant pollen grains enabled us to treat the experimental plants with field recommended rates of pesticides. In a current study we are evaluating the possible mutagenicity of Chicago municipal sewage sludge. We are measuring the frequency of mutant pollen grains in inbred M14 at both the wx-C and wx-90 heteroalleles. These plants were exposed to various concentrations of municipal sewage sludge under field conditions. We have inbred Early-Early Synthetic for five generations and tested this inbred with known mutagens. Early-Early Synthetic is a rapidly maturing inbred growing from kernel to anthesis in approximately 4 weeks and attaining a height of approximately 50 cm. Plants of this inbred have been chronically treated with ethylmethanesulfonate (EMS) or maleic hydrazide (MH) under laboratory conditions and forward mutation at the wx locus was measured in the pollen grains. EMS and MH were mutagenic at concentrations of 1 microM and 10 nM, respectively. The concentrations of EMS and MH were calibrated in Early-Early Synthetic to a linear increase in the frequency of forward mutant pollen grains. The construction of a maize monitor for environmental mutagens is currently in progress. This assay will measure forward or reverse mutation at the wx locus in pollen grains, point mutation in somatic cells and will incorporate a cytogenetic endpoint in root-tip cells.
Authors:
M J Plewa; E D Wagner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental health perspectives     Volume:  37     ISSN:  0091-6765     ISO Abbreviation:  Environ. Health Perspect.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-04-24     Completed Date:  1981-04-24     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0330411     Medline TA:  Environ Health Perspect     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Ethyl Methanesulfonate
Glycogen Synthase / genetics
Maleic Hydrazide
Mutagenicity Tests*
Mutation
Pesticides / analysis*,  pharmacology
Plant Proteins / genetics
Pollen*
Sewage / analysis*
Zea mays / genetics*
Grant Support
ID/Acronym/Agency:
ESO 1895/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Pesticides; 0/Plant Proteins; 0/Sewage; 123-33-1/Maleic Hydrazide; 62-50-0/Ethyl Methanesulfonate; EC 2.4.1.11/Glycogen Synthase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Environ Health Perspect
ISSN: 0091-6765
Article Information
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Print publication date: Month: 1 Year: 1981
Volume: 37First Page: 61 Last Page: 73
ID: 1568634
PubMed Id: 6780335

Germinal cell mutagenesis in specially designed maize genotypes.
M J Plewa
E D Wagner



Article Categories:
  • Research Article


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