| Germ-free C57BL/6J mice are resistant to high-fat-diet-induced insulin resistance and have altered cholesterol metabolism. | |
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MedLine Citation:
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PMID: 20724524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent studies showed that germ-free (GF) mice are resistant to obesity when consuming a high-fat, high-carbohydrate Western diet. However, it remains unclear what mechanisms are involved in the antiobesity phenotype and whether GF mice develop insulin resistance and dyslipidemia with high-fat (HF) feeding. In the present study, we compared the metabolic consequences of HF feeding on GF and conventional (conv) C57BL/6J mice. GF mice consumed fewer calories, excreted more fecal lipids, and weighed significantly less than conv mice. GF/HF animals also showed enhanced insulin sensitivity with improved glucose tolerance, reduced fasting and nonfasting insulinemia, and increased phospho-Akt((Ser-473)) in adipose tissue. In association with enhanced insulin sensitivity, GF/HF mice had reduced plasma TNF-α and total serum amyloid A concentrations. Reduced hypercholesterolemia, a moderate accretion of hepatic cholesterol, and an increase in fecal cholesterol excretion suggest an altered cholesterol metabolism in GF/HF mice. Pronounced nucleus SREBP2 proteins and up-regulation of cholesterol biosynthesis genes indicate that enhanced cholesterol biosynthesis contributed to the cholesterol homeostasis in GF/HF mice. Our results demonstrate that fewer calorie consumption and increased lipid excretion contributed to the obesity-resistant phenotype of GF/HF mice and reveal that insulin sensitivity and cholesterol metabolism are metabolic targets influenced by the gut microbiota. |
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Authors:
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Sylvie Rabot; Mathieu Membrez; Aurélia Bruneau; Philippe Gérard; Taoufiq Harach; Mireille Moser; Frederic Raymond; Robert Mansourian; Chieh J Chou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-19 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 24 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-02 Completed Date: 2011-01-04 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 4948-59 Citation Subset: IM |
Affiliation:
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Institut National de Recherche Agronomique, UMR1319 Micalis, Jouy-en-Josas, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Body Weight / physiology Cholesterol / metabolism* Dietary Fats / adverse effects* Germ-Free Life Glucose Tolerance Test Glycogen / metabolism Insulin Resistance / physiology* Lipids / blood Liver / metabolism Male Mice Mice, Inbred C57BL Obesity / blood, metabolism Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Lipids; 57-88-5/Cholesterol; 9005-79-2/Glycogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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