Document Detail


Germ cell-specific transcriptional regulator sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression.
MedLine Citation:
PMID:  18753606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously discovered a germ cell-specific spermatogenesis and oogenesis basic helix-loop-helix transcription factor, Sohlh2. We generated Sohlh2-deficient mice to understand physiologic consequences of Sohlh2 deletion. We discovered that Sohlh2-knockout adult female mice are infertile due to lack of ovarian follicles. Sohlh2-deficient ovaries can form primordial follicles and, despite limited oocyte growth, do not differentiate surrounding granulosa cells into cuboidal and multilayered structures. Oocytes are rapidly lost in Sohlh2-deficient ovaries, and few are present by 14 days of postnatal life. However, the primordial oocytes are abnormal at the molecular level because they misexpress numerous germ cell- and oocyte-specific genes, including Sohlh1, Nobox, Figla, Gdf9, Pou5f1, Zp1, Zp3, Kit, Oosp1, Nlrp14, H1foo, and Stra8. Our findings show that Sohlh2 is a critical factor for maintenance and differentiation of the oocyte during early oogenesis.
Authors:
Youngsok Choi; Daniel Yuan; Aleksandar Rajkovic
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-08-27
Journal Detail:
Title:  Biology of reproduction     Volume:  79     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-01-06     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1176-82     Citation Subset:  IM    
Affiliation:
Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University, Seoul 135-081, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Basic Helix-Loop-Helix Transcription Factors / biosynthesis*,  genetics*
Cell Differentiation / physiology
Female
Gene Expression / physiology*
Genotype
Germ Cells / physiology*
Granulosa Cells / physiology
Immunohistochemistry
Infertility, Female / genetics
Mice
Mice, Inbred C57BL
Mice, Knockout
Oocytes / metabolism*
Oogenesis / genetics*,  physiology*
Ovarian Follicle / physiology*
RNA / biosynthesis,  isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
HD44858/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Sohlh2 protein, mouse; 63231-63-0/RNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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