Document Detail

Gentamicin negatively influenced osteogenic function in vitro.
MedLine Citation:
PMID:  16710734     Owner:  NLM     Status:  MEDLINE    
Local delivery of gentamicin is an accepted method of infection prophylaxis in the surgery of open fractures. However, the few reports of studies into the effect of locally applied gentamicin on osteoblasts used inadequate methods. In our study, we used the well-characterised C2C12 cell line with reproducible differentiation pathway into the osteoblast lineage. We investigated the viability, cell number, alkaline phosphatase activity, and the expression of osteogenic genes of C2C12 cells after exposure to gentamicin at concentrations of 12.5-800 microg/ml for 48 h. Exposure of C2C12 cells to gentamicin (12.5-800 mg/ml) for 48 h showed no significant changes in the cell number, but cell viability was decreased by one-third at the tested concentrations of 200-800 microg/ml. The alkaline phosphatase activity was significantly decreased by one-third to one-half at any tested concentration (12.5-800 microg/ml) of gentamicin. Any tested concentration of gentamicin up to 800 microg/ml for 48 h did not inhibit or decrease the osteogenic gene expression of osterix and alkaline phosphatase of the C2C12 cells. In conclusion, gentamicin at high concentrations as achieved by local application reduced cellular viability and alkaline phosphatase activity in vitro and therefore may be detrimental for bone healing and repair in vivo.
Akif Ince; Norbert Schütze; Nadja Karl; Jochen F Löhr; Jochen Eulert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-20
Journal Detail:
Title:  International orthopaedics     Volume:  31     ISSN:  0341-2695     ISO Abbreviation:  Int Orthop     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-29     Completed Date:  2007-08-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7705431     Medline TA:  Int Orthop     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  223-8     Citation Subset:  IM    
Department of Orthopaedic Surgery, University Hospital Würzburg, Brettreichstr. 11, 97074 Würzburg, Germany.
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MeSH Terms
Alkaline Phosphatase / metabolism
Cell Line
Cell Survival
Gene Expression
Gentamicins / pharmacology*
Osteogenesis / drug effects*
Polymerase Chain Reaction
Transcription Factors / metabolism
Reg. No./Substance:
0/Gentamicins; 0/Sp7 protein, human; 0/Transcription Factors; EC Phosphatase

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