Document Detail


Gentamicin assay by enzymatic adenylylation and the application of a double osmotic shock procedure to prepare gentamicin adenine mono-nucleotide transferase.
MedLine Citation:
PMID:  339607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The release of gentamicin adenine mono-nucleotide transferase (GAdT) during single cold osmotic shock treatment of E. coli K12 W677/HJR66 is not always maximal. The yield of GAdT could not be improved by using E. coli harvested at different stages of growth, by prolonging the exposure to the different steps of the shock procedure, by changing the sucrose concentration, or the magnesium chloride volume. The quantity of GAdT in osmotic extracts could be increased when a double shock procedure was performed. Using an aliquot (30 microliter) of the extract, an accurate and quick assay for gentamicin, sisomicin and tobramycin in microvolumes of serum (30 microliter) can be accomplished. To avoid high background activity in the assay, the extracts should be prepared from E. coli grown in gentamicin-free medium.
Authors:
J Dankert; J Woltjes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie     Volume:  239     ISSN:  0300-9688     ISO Abbreviation:  Zentralbl Bakteriol Orig A     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1978-02-18     Completed Date:  1978-02-18     Revised Date:  2009-06-05    
Medline Journal Info:
Nlm Unique ID:  0331570     Medline TA:  Zentralbl Bakteriol Orig A     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  113-23     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate
Carbon Radioisotopes
Cold Temperature
Escherichia coli / enzymology
Gentamicins / isolation & purification*
Methods
Nucleotidyltransferases / isolation & purification*
Osmotic Pressure*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Gentamicins; 56-65-5/Adenosine Triphosphate; EC 2.7.7.-/Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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