Document Detail


Genotypic mutation analysis in the p53 gene of benzo[a]pyrene-treated European flounder (Platichthys flesus).
MedLine Citation:
PMID:  10863158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have applied a genotypic mutation detection system (the Restriction Site Mutation (RSM) assay) to detect mutations in the marine teleost flounder (Platichthys flesus). The aim of this study was to evaluate this species as an environmental indicator of genotoxic exposure. We have used the model genotoxin benzo[a]pyrene (B[a]P) to determine the limits of mutation detection in the p53 gene of flounder liver DNA. This study has revealed two important findings. Firstly, we were able to demonstrate that a polymorphism exists in the TaqI restriction site of exon 8 of the flounder p53 gene at codon 243. This polymorphic allele was present as a heterozygote at a mean frequency of 15%, whereas 85% carried the homozygous wild type sequence. Secondly, we established that B[a]P treatment resulted in specific mutational events at the adenine base of the same TaqI site, contrasting previous reports stating that there was a guanine preference for this chemical in mammalian DNA. This difference in mutation specificity may possibly be accounted for by sequence specific factors or by species differences in metabolic activation and/or DNA repair and are worthy of further study.
Authors:
R A Sueiro; G J Jenkins; B P Lyons; J S Harvey; J M Parry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  468     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-19     Completed Date:  2000-07-19     Revised Date:  2008-08-29    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  63-71     Citation Subset:  IM    
Affiliation:
Institute of Food Analysis and Research (IIAA), University of Santiago, Santiago de Compostela, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adenine
Amino Acid Sequence
Amino Acid Substitution
Animals
Base Sequence
Benzo(a)pyrene / toxicity*
DNA / chemistry,  genetics,  metabolism
DNA Mutational Analysis
Deoxyribonucleases, Type II Site-Specific / metabolism
Flounder / genetics*
Genotype
Heterozygote
Mutagenesis, Site-Directed
Mutagenicity Tests / methods
Mutagens / toxicity
Mutation / drug effects
Point Mutation
Polymorphism, Genetic
Time Factors
Tumor Suppressor Protein p53 / genetics*
Chemical
Reg. No./Substance:
0/Mutagens; 0/Tumor Suppressor Protein p53; 50-32-8/Benzo(a)pyrene; 73-24-5/Adenine; 9007-49-2/DNA; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/TCGA-specific type II deoxyribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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