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Genotypic analysis of multiple loci in somatic cells by whole genome amplification.
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MedLine Citation:
PMID:  7567460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.
Authors:
M T Barrett; B J Reid; G Joslyn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  23     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-11-06     Completed Date:  1995-11-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3488-92     Citation Subset:  IM    
Affiliation:
Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*
Alleles
DNA, Neoplasm / genetics*
Esophageal Neoplasms / genetics*
Genetic Markers
Humans
Polymerase Chain Reaction / methods*
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
Sequence Deletion
Grant Support
ID/Acronym/Agency:
CA42792/CA/NCI NIH HHS; CA61202/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Genetic Markers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 9 Year: 1995
Volume: 23 Issue: 17
First Page: 3488 Last Page: 3492
ID: 307228
PubMed Id: 7567460
Publisher Item Identifier: 5a0288

Genotypic analysis of multiple loci in somatic cells by whole genome amplification.
M T Barrett
B J Reid
G Joslyn
Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.



Article Categories:
  • Research Article


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