Document Detail


Genotypic analysis of infant macaques infected transplacentally and orally.
MedLine Citation:
PMID:  8892044     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The SIV-infected macaque provides an excellent model to study factors involved in maternal-fetal transmission of HIV. In our prenatal transmission studies, female macaques were inoculated intravenously during midgestation with either SIV/DeltaB670 or a combination of SIV/ DeltaB670 and the macrophage-tropic molecular clone SIV/17E-Fr. The females harbored a genetically diverse virus population at parturition, whereas a single genotype from the maternal quasispecies was identified in the infants. One of two variants was transplacentally transmitted to the infants, SIV/17E-Fr or B670-Cl 12, a genotype contained within the SIV/ DeltaB670 inoculum. Both of these variants have been identified in the central nervous system of macaques that have developed encephalitis and they replicate in vitro on primary rhesus macrophages. These results suggest a critical role for macrophages in fetal infection in utero. In our perinatal transmission studies we have evaluated the viral genotypes found in two newborn macaques infected orally with SIV/DeltaB670 and in one infant infected via amniotic inoculation in late gestation. More than one viral genotype was identified in each infant, moreover, each infant harbored different genotypes. These results suggest different mechanisms are responsible for viral infection via these routes.
Authors:
A Martin Amedee; N Lacour; L N Martin; J E Clements; R B Bohm; B Davison; R Harrison; M Murphey-Corb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medical primatology     Volume:  25     ISSN:  0047-2565     ISO Abbreviation:  J. Med. Primatol.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-02-06     Completed Date:  1997-02-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0320626     Medline TA:  J Med Primatol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  225-35     Citation Subset:  IM; X    
Affiliation:
Tulane Regional Primate Research Center, Covington, LA 70433, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
DNA Primers
Female
Genes, env
Genetic Variation
Genotype
Infectious Disease Transmission, Vertical
Macaca mulatta
Models, Biological
Molecular Sequence Data
Placenta
Polymerase Chain Reaction
Pregnancy
Pregnancy Complications, Infectious*
Sequence Homology, Amino Acid
Simian Acquired Immunodeficiency Syndrome / embryology,  transmission*,  virology
Simian immunodeficiency virus / genetics*,  isolation & purification
Viral Envelope Proteins / chemistry,  genetics*
Grant Support
ID/Acronym/Agency:
AI32302/AI/NIAID NIH HHS; RR00164/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Viral Envelope Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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