Document Detail


Genotypes, exotoxin gene content, and antimicrobial resistance of Staphylococcus aureus strains recovered from foods and food handlers.
MedLine Citation:
PMID:  22327598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcal food poisoning, one of the most common food-borne diseases, results from ingestion of one or more staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus in foods. In the present study, 64 S. aureus isolates recovered from foods and food handlers, associated or not associated with food-poisoning outbreaks in Spain, were investigated. They were assigned to 31 strains by spa typing, multilocus sequence typing (MLST), exotoxin gene content, and antimicrobial resistance. The strains belonged to 10 clonal complexes (CCs): CC5 (29.0%), CC30 (25.8%), CC45 (16.1%), CC8, CC15 (two strains each), CC1, CC22, CC25, CC59, and CC121 (one strain each). They contained hemolysin genes (90.3%); lukED (77.4%); exfoliatin genes eta, etd (6.5% each), and etb (3.2%); tst (25.8%); and the following enterotoxin or enterotoxin-like genes or clusters: sea (38.7%), seb (12.9%), sec (16.1%), sed-selj with or without ser (22.9%), selk-selq (6.5%), seh, sell, selp (9.7% each), egc1 (32.3%), and egc2 (48.4%). The number of se and sel genes ranged from zero to 12. All isolates carrying tst, and most isolates with genes encoding classical enterotoxins (SEA, SEB, SEC, and SED), expressed the corresponding toxin(s). Two CC5 isolates from hamburgers (spa type t002, sequence type 5 [ST5]; spa type t2173, ST5) were methicillin resistant and harbored staphylococcal cassette chromosome mec (SCCmec) IVd. Six (19.4%) were mupirocin resistant, and one (spa type t120, ST15) from a food handler carried mupA (MIC, 1,250 μg/ml). Resistance to ampicillin (blaZ) (61.3%), erythromycin (ermA-ermC or ermC) (25.8%), clindamycin (msrA-msrB or msrB) (16.1%), tetracycline (tetK) (3.2%), and amikacin-gentamicin-kanamycin-tobramycin (aphA with aacA plus aphD or aadD) (6.5%) was also observed. The presence of S. aureus strains with an important repertoire of virulence and resistance determinants in the food chain represents a potential health hazard for consumers and merits further observation.
Authors:
M A Argudín; M C Mendoza; M A González-Hevia; M Bances; B Guerra; M R Rodicio
Related Documents :
2220838 - Chronic neuropsychological sequelae of occupational exposure to organophosphate insecti...
1639938 - Food contains the bioactive peptide, cyclo(his-pro).
24103458 - Characteristics of fast-food/takeaway-food and restaurant/café-food consumers among ne...
8475078 - The nucleotide in position 32 of the trna anticodon loop determines ability of anticodo...
6462548 - Effects of restricted dietary flavor experience before weaning on postweaning food pref...
24695888 - Ventral frontal satiation-mediated responses to food aromas in obese and normal-weight ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-10
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-26     Completed Date:  2012-07-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2930-5     Citation Subset:  IM    
Affiliation:
Department of Functional Biology, Microbiology Area, University of Oviedo, Oviedo, Spain. rrodicio@fq.uniovi.es
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Toxins / genetics
Carrier State / microbiology*
Cluster Analysis
Drug Resistance, Bacterial*
Exotoxins / genetics*
Food Microbiology*
Genotype
Molecular Typing
Spain
Staphylococcal Infections / microbiology*
Staphylococcus aureus / classification*,  drug effects*,  genetics
Virulence Factors / genetics
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Exotoxins; 0/Virulence Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Key residues for controlling enantioselectivity of Halohydrin dehalogenase from Arthrobacter sp. str...
Next Document:  Prevalence of Yersinia enterocolitica in pigs slaughtered in Chinese abattoirs.