| Genotype and mating type distribution within clinical Cryptococcus neoformans and Cryptococcus gattii isolates from patients with cryptococcal meningitis in Uberaba, Minas Gerais, Brazil. | |
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MedLine Citation:
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PMID: 19905964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We molecularly characterized 81 cryptococcal isolates recovered from cerebrospinal fluid samples of 77 patients diagnosed between 1998 and 2007 as having cryptococcal meningitis in Uberaba Minas Gerais, Brazil. Fifty-seven (74%) were male with a mean age 35.6 years. Seventy-two (88.9%) of the isolates were from 68 AIDS patients and cryp-tococcosis was the first AIDS-defining condition in 38 (55.9%) patients. Cryptococcosis and AIDS were simultaneously diagnosed in 25 (65.8%) of these 38 patients. Genotypes were characterized through the use of URA5 restriction fragment length polymorphisms analysis, the genetic variability was determined using PCR-fingerprinting with the minisatellite-specific primer M13, and the mating type and serotypes were established by PCR. Seventy-six of the 81 isolates were Cryptococcus neoformans (93.8%), while the remaining five were C. gattii (6.1%), but all were mating type alpha. C. neoformans isolates were genotype VNI (serotype A), while C. gattii isolates were VGII. Four of the latter isolates were identical, but only two were from AIDS patients. Six of the nine isolates from non-AIDS patients were VNI. PCR fingerprints of the isolates from two of the three AIDS patients with clinical relapse were 100% identical. The predominance of VNI and mating type alpha is in accordance with data from other parts of the world. The occurrence of VGII in Minas Gerais indicates a geographical expansion within Brazil. |
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Authors:
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Delio José Mora; André Luiz Pedrosa; Virmondes Rodrigues; Claudia Maria Leite Maffei; Luciana Trilles; Márcia Dos Santos Lazéra; Mario León Silva-Vergara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical mycology : official publication of the International Society for Human and Animal Mycology Volume: 48 ISSN: 1460-2709 ISO Abbreviation: Med. Mycol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-14 Completed Date: 2010-07-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815835 Medline TA: Med Mycol Country: England |
Other Details:
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Languages: eng Pagination: 561-9 Citation Subset: IM |
Affiliation:
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Disciplina de Doenças Infecciosas e Parasitárias, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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AIDS-Related Opportunistic Infections
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microbiology Adolescent Adult Brazil Cryptococcus gattii / genetics*, isolation & purification Cryptococcus neoformans / genetics*, isolation & purification DNA Fingerprinting Female Genes, Mating Type, Fungal / genetics* Genotype Humans Male Meningitis, Cryptococcal / microbiology* Middle Aged Polymerase Chain Reaction Young Adult |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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