Document Detail


Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay.
MedLine Citation:
PMID:  19595757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Higher throughput methods, high content analysis and automated screening methods are of highest demand in drug development today. In toxicology, these strategies are becoming increasingly important, as well. Therefore, an integrated higher throughput method for the comet assay is addressed by the development presented here. The sensitivity, specificity and relevance of the comet assay as a method for determination of DNA damage in vivo and in vitro have been highlighted in many studies. Actually, efforts are made to include it in a panel of genotoxicity tests for regulatory purposes. However, the standard comet assay is a time consuming procedure due to the specific methods needed. The improvements presented here lead to a faster and easier slide-production, a smaller amount of cells needed, a higher amount of comets quantified, a fully automated analysis of comets including reanalysis, storing, visualisation and documentation possibilities using standard comet quantification models such as tail length or tail moment, and - by introduction of clearly definable selection criteria based on image analysis algorithms - clearly improve objectivity and standardization of the analysis procedure. Results prove the high reproducibility, flexibility, efficiency and suitability of the procedure as a fully automated analysis method in higher throughput genotoxicity testing in vitro.
Authors:
Detlef Ritter; Jan Knebel
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Publication Detail:
Type:  Journal Article     Date:  2009-07-10
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  23     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-13     Completed Date:  2010-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  1570-5     Citation Subset:  IM    
Affiliation:
Fraunhofer Institute Toxicology and Experimental Medicine, Nikolai-Fuchs-Str. 1, D-30625 Hannover, Germany. detlef.ritter@item.fraunhofer.de
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MeSH Terms
Descriptor/Qualifier:
Comet Assay / methods*
DNA Damage
High-Throughput Screening Assays / methods*
Humans
Image Processing, Computer-Assisted

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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