| Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay. | |
| | |
MedLine Citation:
|
PMID: 19595757 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Higher throughput methods, high content analysis and automated screening methods are of highest demand in drug development today. In toxicology, these strategies are becoming increasingly important, as well. Therefore, an integrated higher throughput method for the comet assay is addressed by the development presented here. The sensitivity, specificity and relevance of the comet assay as a method for determination of DNA damage in vivo and in vitro have been highlighted in many studies. Actually, efforts are made to include it in a panel of genotoxicity tests for regulatory purposes. However, the standard comet assay is a time consuming procedure due to the specific methods needed. The improvements presented here lead to a faster and easier slide-production, a smaller amount of cells needed, a higher amount of comets quantified, a fully automated analysis of comets including reanalysis, storing, visualisation and documentation possibilities using standard comet quantification models such as tail length or tail moment, and - by introduction of clearly definable selection criteria based on image analysis algorithms - clearly improve objectivity and standardization of the analysis procedure. Results prove the high reproducibility, flexibility, efficiency and suitability of the procedure as a fully automated analysis method in higher throughput genotoxicity testing in vitro. |
| | |
Authors:
|
Detlef Ritter; Jan Knebel |
Related Documents
:
|
20864567 - A comparison of the sensitivity, specificity, and molecular weight accuracy of three di... 3300727 - Radioenzymatic microassay for picogram quantities of serotonin or acetylserotonin in bi... 22284887 - Applicability of accelerated solvent extraction for synthetic colorants analysis in mea... 3159337 - Quantitation of ciprofloxacin in body fluids by high-pressure liquid chromatography. 16492397 - Determination of benzethonium chloride in anthrax vaccine adsorbed by hplc. 10327627 - Study of nitrophenols preconcentration using quinolin-8-ol immobilized on controlled-po... |
Publication Detail:
|
Type: Journal Article Date: 2009-07-10 |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 23 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-11-13 Completed Date: 2010-01-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
|
Languages: eng Pagination: 1570-5 Citation Subset: IM |
Affiliation:
|
Fraunhofer Institute Toxicology and Experimental Medicine, Nikolai-Fuchs-Str. 1, D-30625 Hannover, Germany. detlef.ritter@item.fraunhofer.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Comet Assay
/
methods* DNA Damage High-Throughput Screening Assays / methods* Humans Image Processing, Computer-Assisted |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Age-related increase of prostaglandin D(2) synthase concentration and glycation in ovine cerebrospin...
Next Document: Study of cytotoxic effect of photodynamically and sonodynamically activated sensitizers in vitro.