Document Detail


Genotoxicity assessment using comet assay in Behcet's disease patients.
MedLine Citation:
PMID:  16019038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Behcet's disease (BD) was originally described by Turkish dermatologist, Hulusi Behcet in 1937. BD is an inflammatory disorder of unknown cause, characterized by recurrent oral aphthous ulcers, genital ulcers, uveitis, and skin lesions. All these common manifestations are self-limiting except for ocular attacks. The aims of this study were to assess whether BD patients have more genotoxicity than healthy controls and whether colchicine (COL) treated BD patients are different from those not using COL in terms of genotoxicity. A few dozens of methods have been developed and used for the assessment of genotoxicity. The most popular method is based on single cell gel electrophoresis (COMET assay) in alkaline condition. After electrophoresis, captured images are subjected to digital image analysis to find the values for percent tail DNA from comet assay parameters consistent with genotoxicity. COMET assay was performed in isolated lymphocytes from 42 COL treated Behcet's disease patients, 9 BD patients not using COL, and 36 healthy controls. In the COL-BD patients and non-COL-BD patients, the mean age (range 14-56 years) and mean disease duration (range 0.5-24 years) did not differ between the two groups. We found statistical differences in percent tail DNA between BD and the healthy controls (13.38+/-9.58 versus 2.77+/-1.45, P<0.0001). No difference in percent tail DNA was observed between users and non-users of COL, whereas it was more different in inactive BD patients than active ones (19.75+/-10.49 versus 11.83+/-8.79, P<0.05, respectively). Genotoxicity, as assessed by COMET assay, is increased in BD patients. These results suggest that genotoxicity is associated with BD itself rather than COL use.
Authors:
Birsen Bilgici; Abdulkerim Bedir; Nilgün Sentürk; Muhlise Alvur; Fatma Aydin; Ahmet Yasar Turanli
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Publication Detail:
Type:  Journal Article     Date:  2005-07-12
Journal Detail:
Title:  Mutation research     Volume:  578     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2005-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  170-4     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Medicine, Ondokuz Mayis University, 55139 Samsun, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Behcet Syndrome / genetics*
Case-Control Studies
Colchicine / toxicity
Comet Assay*
DNA Damage / drug effects*
Female
Humans
Male
Middle Aged
Mutagenicity Tests*
Mutagens / toxicity
Turkey
Chemical
Reg. No./Substance:
0/Mutagens; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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