| Genotoxicity of the Yamuna River water at Okhla (Delhi), India. | |
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MedLine Citation:
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PMID: 15922807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Water samples from the Yamuna River at Okhla (Delhi), India, were concentrated using XAD resins (XAD-4 and XAD-8) and liquid-liquid extraction procedures. Gas chromatographic analysis of liquid-liquid extracted water samples revealed the presence of the pesticides DDT, BHC, dieldrin, endosulfan, aldrin, 2,4-D, dimethoate, methyl parathion, and malathion at concentrations of 14, 25, 2.1, 114, 0.9, 0.6, 0.9, 1.7, and 1.9 ng/L, respectively. The genotoxicity of the extracted water samples was evaluated with the Ames Salmonella/mammalian microsome test, DNA repair-defective mutants, and bacteriophage lambda systems. The results of the Salmonella test demonstrated that the XAD-concentrated water samples had maximum mutagenicity with the TA98 strain both with and without metabolic activation. However, the liquid-liquid-extracted water samples were also found to be mutagenic with one or more of the Ames tester strains, but to a lesser extent as compared with XAD extracts. The damage brought about in the DNA repair-defective mutants in the presence of XAD-concentrated water samples was found to be markedly high as compared with that liquid-liquid-extracted water samples at the dose level of 20 microl/mL culture. All mutants invariably exhibited significant declines in their colony-forming units as compared with their isogenic wild-type counterparts. Survival decreased by 86.3 and 75.5% in the polA- strain after 6 h of treatment with XAD-concentrated and liquid-liquid-extracted water samples, respectively. A significant decrease was also observed in the survival of bacteriophage lambda when treated with the test samples. Mutagenic responses of the liquid-liquid-extracted water samples may not necessarily reflect the mutagenicity of existing pesticides in the test water, because some other organic pollutants might accompany the pesticides in the extract. |
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Authors:
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Asma Aleem; Abdul Malik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Ecotoxicology and environmental safety Volume: 61 ISSN: 0147-6513 ISO Abbreviation: Ecotoxicol. Environ. Saf. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-05-30 Completed Date: 2005-10-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7805381 Medline TA: Ecotoxicol Environ Saf Country: United States |
Other Details:
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Languages: eng Pagination: 404-12 Citation Subset: IM |
Affiliation:
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Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202 002, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gas DNA Damage* DNA Repair India Mammals Microsomes Mutagenicity Tests Pesticides / toxicity* Rivers Salmonella Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Pesticides; 0/Water Pollutants, Chemical |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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