Document Detail


Genotoxicity Assessment of Amaranth and Allura Red Using Saccharomyces Cerevisiae.
MedLine Citation:
PMID:  23132362     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Amaranth (E123) and Allura red (E129), very important food azo dyes used in food, drug, paper, cosmetic and textile industries, were assessed for their genotoxic potential through comet assay in yeast cells. Comet assay was standardized by with different concentration of H(2)O(2). Concentrations of Amaranth and Allura red were maintained in sorbitol buffer starting from 9.76 to 5,000 μg/mL and 1 × 10(4) cells were incubated at two different incubation temperatures 28 and 37°C. Amaranth (E123) and Allura red (E129) were found to exhibit their genotoxic effect directly in Saccharomyces cerevisiae. No significant genotoxic activity was observed for Amaranth and Allura red at 28°C but at 37°C direct relation of Amaranth concentration with comet tail was significant and no positive relation was seen with time exposure factor. At 37°C the minimum concentration of Amaranth and Allura red at which significant DNA damage observed through comet assay was 1,250 μg/mL in 2nd h post exposure time. The results indicated that food colors should be carefully used in baking products as heavy concentration of food colors could affect the fermentation process of baking.
Authors:
Hafiza Sumara Jabeen; Sajjad Ur Rahman; Shahid Mahmood; Sadaf Anwer
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-7
Journal Detail:
Title:  Bulletin of environmental contamination and toxicology     Volume:  -     ISSN:  1432-0800     ISO Abbreviation:  Bull Environ Contam Toxicol     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0046021     Medline TA:  Bull Environ Contam Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan, Sumara.jabeen@gmail.com.
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