Document Detail


Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells.
MedLine Citation:
PMID:  22847138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythrosine (ErB) is a xanthene and an US Food and Drug Administration approved dye used in foods, drugs and cosmetics. Although its utilization is permitted, ErB is described as inhibitor of enzymes and protein-protein interactions and is toxic to pituitary and spermatogenesis processes. However, the genotoxicity and mutagenicity of ErB is inconclusive in the literature. This study aimed to analyze the genotoxicity of this dye using the alkaline comet assay and is the first investigation to evaluate ErB mutagenicity using the cytokinesis block micronucleus cytome (CBMN-Cyt) assay in HepG2 cells. These cells were chosen because they produce phase I and phase II enzymes that can mimic in vivo metabolism. The cells were treated with seven concentrations (0.1-70.0 μg mL(-1)) of ErB, and the results showed genotoxicity at the two highest concentrations and mutagenicity at six concentrations. Furthermore, as micronuclei result from clastogenic and aneugenic processes, while comet assay is often considered more sensitive and detects DNA single strain breaks, we suggest that an aneugenic is responsible for the observed damage. Although ErB is approved for use in the food, cosmetic and pharmaceutical industries, it must be used carefully because it damages the DNA structure.
Authors:
Farah Maria Drumond Chequer; Vinícius de Paula Venâncio; Maria de Lourdes Pires Bianchi; Lusânia Maria Greggi Antunes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-28
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  50     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-24     Completed Date:  2013-03-06     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3447-51     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP 14040-903, Brazil. farahchequer@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / drug effects
DNA Damage
Erythrosine / chemistry,  toxicity*
Hep G2 Cells
Humans
Micronucleus Tests
Molecular Structure
Mutagenesis / drug effects*
Chemical
Reg. No./Substance:
16423-68-0/Erythrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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