Document Detail

Genotoxic effects of myosmine in human lymphocytes and upper aerodigestive tract epithelial cells.
MedLine Citation:
PMID:  14580784     Owner:  NLM     Status:  MEDLINE    
Myosmine, 3-(1-pyrroline-2-yl)pyridine, is an alkaloid found in tobacco plants. Recently, it was also detected in various edibles and staple foods. Whereas other tobacco alkaloids such as nicotine and nornicotine and their nitrosation products, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN), have been widely discussed, the mutagenic impact of myosmine has not been investigated in detail. In the present study, possible genotoxic effects of myosmine were studied in human lymphocytes and nasal mucosal cells using the alkaline single cell microgel electrophoresis (Comet) assay. DNA single strand breaks, alkali labile sites and incomplete excision repair sites were expressed using the Olive tail moment (OTM). One hour incubation with myosmine at 0, 5, 10, 25 and 50 mM induced a low but significantly dose-dependent increase of DNA migration from 1.29 +/- 0.13 to 18.25 +/- 1.59 (OTM, mean +/- S.E., N=11) in lymphocytes. In nasal mucosal cells a similar although somewhat less extensive DNA damage from 1.17 +/- 0.12 to 21.67 +/- 2.97 (OTM, mean +/- S.E., N=10-11) was obtained after 1 h incubation with myosmine at 0, 10, 25, 50 and 100 mM. After prolonged incubation of human lymphocytes with 10mM myosmine for 1, 3, 6, and 24 h, a significant time-dependent increase of DNA migration from 3.45 +/- 0.43 to 57.77 +/- 8.24 (OTM, mean +/- S.E., N=4) was observed. Our data indicate that myosmine expresses significant genotoxic effects in human target cells of carcinogenesis. This result warrants further investigations on the impact of this dietary component on human health.
Norbert H Kleinsasser; Barbara C Wallner; Ulrich A Harréus; Wolfgang Zwickenpflug; Elmar Richter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology     Volume:  192     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-28     Completed Date:  2003-12-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  171-7     Citation Subset:  IM    
Klinisch experimentelle Onkologie, Department of Otolaryngology-Head and Neck Surgery, Ludwig-Maximilians-University of Munich, Pettenkoferstr. 4a, D-80336 Munich, Germany.
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MeSH Terms
Alkaloids / toxicity*
Cells, Cultured
Comet Assay
DNA Damage*
Dose-Response Relationship, Drug
Epithelial Cells / drug effects*
Lymphocytes / drug effects*
Middle Aged
Mutagens / toxicity*
Nasal Mucosa / cytology,  drug effects
Reg. No./Substance:
0/Alkaloids; 0/Mutagens; 532-12-7/myosmine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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