| Genotoxic effects of aluminum chloride in cultured human lymphocytes treated in different phases of cell cycle. | |
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MedLine Citation:
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PMID: 17321660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aluminum (Al) is the most abundant metal and the third common chemical element on earth. It is known that Al is toxic, especially its trivalent form (Al(3+)), that represents the its most soluble form. Al intoxication is related to some pathogenic disorders, principally neurodegeneratives ones as Parkinson and Alzheimer diseases. The present study aimed to evaluate the mutagenic potential of aluminum chloride (AlCl(3)). Comet assay and chromosome aberrations analysis were applied to evaluate the DNA-damaging and clastogenic effects of AlCl(3), respectively, in different phases of the cell cycle. Cultured human lymphocytes were treated with 5, 10, 15 and 25 microM aluminum chloride during the G1, G1/S, S (pulses of 1 and 6h), and G2 phases of the cell cycle. All tested concentrations were cytotoxic and reduced significantly the mitotic index in all phases of cell cycle. They also induced DNA damage and were clastogenic in all phases of cell cycle, specially in S phase. AlCl(3) also induced endoreduplication and polyploidy in treatments performed during G1 phase. The presence of genotoxicity and polyploidy on interphase and mitosis, respectively, suggests that aluminum chloride is clastogenic and indirectly affects the construction of mitotic fuse in all tested concentrations. |
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Authors:
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P D L Lima; D S Leite; M C Vasconcellos; B C Cavalcanti; R A Santos; L V Costa-Lotufo; C Pessoa; M O Moraes; R R Burbano |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-11 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 45 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-04-23 Completed Date: 2007-07-25 Revised Date: 2008-06-03 |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 1154-9 Citation Subset: IM |
Affiliation:
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Human Cytogenetics Laboratory, Department of Biology, Center for Biological Sciences, Federal University of Pará, Belém/PA, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aluminum Compounds
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toxicity* Aneugens / toxicity* Cell Cycle / drug effects* Cell Survival / drug effects Cells, Cultured Chlorides / toxicity* Chromosome Aberrations Comet Assay DNA Damage Dose-Response Relationship, Drug Environmental Pollutants / toxicity* Humans Lymphocytes / drug effects*, pathology Polyploidy |
| Chemical | |
Reg. No./Substance:
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0/Aluminum Compounds; 0/Aneugens; 0/Chlorides; 0/Environmental Pollutants; 7446-70-0/aluminum chloride |
| Comments/Corrections | |
Comment In:
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Food Chem Toxicol. 2008 Mar;46(3):1204; author reply 1205
[PMID:
18054421
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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