Document Detail


Genotoxic effects of aluminum chloride in cultured human lymphocytes treated in different phases of cell cycle.
MedLine Citation:
PMID:  17321660     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aluminum (Al) is the most abundant metal and the third common chemical element on earth. It is known that Al is toxic, especially its trivalent form (Al(3+)), that represents the its most soluble form. Al intoxication is related to some pathogenic disorders, principally neurodegeneratives ones as Parkinson and Alzheimer diseases. The present study aimed to evaluate the mutagenic potential of aluminum chloride (AlCl(3)). Comet assay and chromosome aberrations analysis were applied to evaluate the DNA-damaging and clastogenic effects of AlCl(3), respectively, in different phases of the cell cycle. Cultured human lymphocytes were treated with 5, 10, 15 and 25 microM aluminum chloride during the G1, G1/S, S (pulses of 1 and 6h), and G2 phases of the cell cycle. All tested concentrations were cytotoxic and reduced significantly the mitotic index in all phases of cell cycle. They also induced DNA damage and were clastogenic in all phases of cell cycle, specially in S phase. AlCl(3) also induced endoreduplication and polyploidy in treatments performed during G1 phase. The presence of genotoxicity and polyploidy on interphase and mitosis, respectively, suggests that aluminum chloride is clastogenic and indirectly affects the construction of mitotic fuse in all tested concentrations.
Authors:
P D L Lima; D S Leite; M C Vasconcellos; B C Cavalcanti; R A Santos; L V Costa-Lotufo; C Pessoa; M O Moraes; R R Burbano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-11
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  45     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-04-23     Completed Date:  2007-07-25     Revised Date:  2008-06-03    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1154-9     Citation Subset:  IM    
Affiliation:
Human Cytogenetics Laboratory, Department of Biology, Center for Biological Sciences, Federal University of Pará, Belém/PA, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Aluminum Compounds / toxicity*
Aneugens / toxicity*
Cell Cycle / drug effects*
Cell Survival / drug effects
Cells, Cultured
Chlorides / toxicity*
Chromosome Aberrations
Comet Assay
DNA Damage
Dose-Response Relationship, Drug
Environmental Pollutants / toxicity*
Humans
Lymphocytes / drug effects*,  pathology
Polyploidy
Chemical
Reg. No./Substance:
0/Aluminum Compounds; 0/Aneugens; 0/Chlorides; 0/Environmental Pollutants; 7446-70-0/aluminum chloride
Comments/Corrections
Comment In:
Food Chem Toxicol. 2008 Mar;46(3):1204; author reply 1205   [PMID:  18054421 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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