Document Detail


Genomic restriction map of the extremely thermophilic bacterium Thermus thermophilus HB8.
MedLine Citation:
PMID:  8416889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A physical map of the chromosome of the extremely thermophilic eubacterium Thermus thermophilus HB8 has been constructed by using pulsed-field gel electrophoresis techniques. A total of 26 cleavage sites for the rarely cutting restriction endonucleases HpaI, MunI, and NdeI were located on the genome. On the basis of the sizes of the restriction fragments generated, the genome size was estimated to be 1.74 Mbp, which is significantly smaller than the chromosomes of Escherichia coli and other mesophiles. Partial digestion experiments revealed the order of the six HpaI bands on the chromosome. Hybridization of isolated restriction fragments to pulsed-field gel-separated restriction digestions confirmed the deduced order of the HpaI fragments and allowed ordering and alignment of the NdeI and MunI fragments. In addition, 16 genes or gene clusters cloned from several different Thermus strains were located on the T. thermophilus HB8 chromosomal map by hybridization of gene probes to pulsed-field gel-resolved restriction digestions.
Authors:
K M Borges; P L Bergquist
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  175     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-01-27     Completed Date:  1993-01-27     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-10     Citation Subset:  IM    
Affiliation:
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Cloning, Molecular
DNA, Bacterial / genetics*
Deoxyribonucleases, Type II Site-Specific / metabolism
Electrophoresis, Gel, Pulsed-Field
Genes, Bacterial / genetics
Genome, Bacterial*
Molecular Weight
Multigene Family / genetics
Nucleic Acid Hybridization
Restriction Mapping
Thermus thermophilus / genetics*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; EC 3.1.21.-/endodeoxyribonuclease HpaI; EC 3.1.21.-/endodeoxyribonuclease MfeI; EC 3.1.21.-/endodeoxyribonuclease NdeI; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific
Comments/Corrections

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