Document Detail


Genomic organization and chromosomal localization of a member of the MAP kinase phosphatase gene family to human chromosome 11p15.5 and a pseudogene to 10q11.2.
MedLine Citation:
PMID:  9192849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mitogen-activated protein kinase phosphatases (MKPs) play a central role in a variety of signaling pathways. We recently described a novel murine MKP, M3/6, which is uniquely specific for c-Jun N-terminal kinase/stress-activated protein kinase and p38 kinase. Here we report the localization of the human orthologue of this gene, HB5, to within 150 kb of H19 on human chromosome 11p15.5. The gene consists of six exons. Two of the introns in HB5 are not found in other genes of this family, suggesting an evolutionary split between MKPs displaying specificity toward different MAP kinases. An intronless pseudogene is present on chromosome 10q11.2. Although 11p15.5 is an imprinted region, HB5 is almost entirely unmethylated on both alleles in lymphocytes. Chromosome 11p15 has been implicated in the development of a number of tumor types, including lung, a tissue known to express this gene. Loss of heterozygosity was found in one of eight informative lung tumors studied.
Authors:
M A Nesbit; M D Hodges; L Campbell; T M de Meulemeester; M Alders; N R Rodrigues; K Talbot; A M Theodosiou; M A Mannens; Y Nakamura; P F Little; K E Davies
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  42     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-09-02     Completed Date:  1997-09-02     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  284-94     Citation Subset:  IM    
Affiliation:
Genetics Laboratory, Department of Biochemistry, University of Oxford, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Animals
Base Sequence
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Chromosome Mapping
Chromosomes, Human, Pair 10 / genetics*
Chromosomes, Human, Pair 11 / genetics*
Cloning, Molecular
DNA Methylation
DNA Primers / genetics
DNA, Complementary / genetics,  isolation & purification
Exons
Humans
In Situ Hybridization, Fluorescence
Introns
Lung Neoplasms / genetics
Mice
Molecular Sequence Data
Multigene Family
Polymerase Chain Reaction
Polymorphism, Genetic
Protein Tyrosine Phosphatases / genetics*,  metabolism
Pseudogenes
Substrate Specificity
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 3.1.3.48/Protein Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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