Document Detail


Genomic instability induced by ionizing radiation in human hepatocytes.
MedLine Citation:
PMID:  22757674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to characterize genomic instability induced by ionizing radiation (IR) in human hepatocytes as reflected by alterations in cloning efficiency, micronucleus (MN) frequency, and apoptosis. The human normal liver 7702 cell line (HL7702) was subjected to initial irradiation of (60)Co-γ ray at doses of 0 (control group), 2, 4, 6, 8, or 10 Gy in each group. Progeny of surviving cells from a second irradiation at dose of 2 Gy were cultured for 15 passages until they were transferred. The cloning efficiency, MN frequency, and apoptotic rate were measured after the initial irradiation, and repeated at passage 15 before and after the second irradiation. The initial irradiation resulted in a dose-dependent decline in cloning efficiency and an increase in MN frequency and apoptotic rate. At passage 15 in progeny of initially irradiated cells, cloning efficiency, MN frequency returned to control levels while apoptotic rate rose. After the second irradiation, cloning efficiency fell while a rise in MN frequency and apoptosis occurred. Our results show that the second irradiation may further enhance cell progeny injury induced by initial irradiation, such that genomic instability that may be difficult to detect after one irradiation is more apparent with subsequent irradiation.
Authors:
Ya-Hui Zuo; Xu-Hong Dang; Hui-Fang Zhang; Jian-Gong Liu; Zhi-Kai Duan; Zhong-Wen Wang; Jian Tong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  75     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-04     Completed Date:  2012-09-11     Revised Date:  2012-12-03    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  700-6     Citation Subset:  IM    
Affiliation:
Division of Radiology and Environmental Medicine, China Institute for Radiation Protection, Taiyuan, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / radiation effects
Cell Line
Cloning, Molecular / radiation effects
Dose-Response Relationship, Radiation
Gamma Rays / adverse effects*
Genomic Instability / radiation effects*
Hepatocytes / radiation effects*
Humans
Micronuclei, Chromosome-Defective / radiation effects
Micronucleus Tests

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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