Document Detail

Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats.
MedLine Citation:
PMID:  18670894     Owner:  NLM     Status:  MEDLINE    
This study was undertaken to investigate the genomic instability on blood cells during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis by means of single cell gel (comet) and micronucleus assays. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Ten animals were used as negative control. Although no histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, genetic damage was found in blood cells as depicted by the mean tail moment and an increase of micronucleated polychromatic erythrocytes. After 12 and 20 weeks treatment, the same picture occurred, being the strong effect observed in the micronucleus induction. These periods correspond to pre-neoplastic lesions and well-differentiated squamous cell carcinomas, respectively. Taken together, our results support the idea that genomic instability on blood cells appears to be associated with the risk and progression of oral cancer, being a reliable tool for detecting early systemic conditions of malignancy.
Daniel A Ribeiro; Daniela G Grilli; Daisy M F Salvadori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-01
Journal Detail:
Title:  Journal of molecular histology     Volume:  39     ISSN:  1567-2379     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2009-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  481-6     Citation Subset:  IM    
Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Av. Ana Costa, 95, Vila Mathias, Santos, SP, 11060-001, Brazil.
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MeSH Terms
4-Nitroquinoline-1-oxide / toxicity*
Carcinogens / toxicity*
Comet Assay*
Erythroblasts / metabolism*,  pathology
Erythrocytes, Abnormal / metabolism*,  pathology
Genomic Instability / drug effects*
Micronuclei, Chromosome-Defective / chemically induced
Predictive Value of Tests
Rats, Wistar
Time Factors
Tongue Neoplasms / chemically induced*,  metabolism*,  pathology
Reg. No./Substance:
0/Carcinogens; 56-57-5/4-Nitroquinoline-1-oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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