Document Detail


Genomic and gene variation in Mycoplasma hominis strains.
MedLine Citation:
PMID:  2444564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNAs from 14 strains of Mycoplasma hominis isolated from various habitats, including strain PG21, were analyzed for genomic heterogeneity. DNA-DNA filter hybridization values were from 51 to 91%. Restriction endonuclease digestion patterns, analyzed by agarose gel electrophoresis, revealed no identity or cluster formation between strains. Variation within M. hominis rRNA genes was analyzed by Southern hybridization of EcoRI-cleaved DNA hybridized with a cloned fragment of the rRNA gene from the mycoplasma strain PG50. Five of the M. hominis strains showed identical hybridization patterns. These hybridization patterns were compared with those of 12 other mycoplasma species, which showed a much more complex band pattern. Cloned nonribosomal RNA gene fragments of M. hominis PG21 DNA were analyzed, and the fragments were used to demonstrate heterogeneity among the strains. A monoclonal antibody against strain PG21 was produced. The possible presence of the corresponding antigenic epitope in the other 13 M. hominis strains was analyzed by immunoblotting and compared with results of immunoblotting using polyclonal antibodies against PG21.
Authors:
G Christiansen; H Andersen; S Birkelund; E A Freundt
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Israel journal of medical sciences     Volume:  23     ISSN:  0021-2180     ISO Abbreviation:  Isr. J. Med. Sci.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-12-15     Completed Date:  1987-12-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0013105     Medline TA:  Isr J Med Sci     Country:  ISRAEL    
Other Details:
Languages:  eng     Pagination:  595-602     Citation Subset:  IM    
Affiliation:
Institute of Medical Microbiology, University of Aarhus, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial / immunology
Antibodies, Monoclonal / immunology
Antigens, Bacterial / genetics
DNA, Bacterial / genetics
Epitopes / genetics
Genes, Bacterial*
Genetic Variation
Mycoplasma / classification,  genetics*,  immunology
Nucleic Acid Hybridization
RNA, Bacterial / genetics
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal; 0/Antigens, Bacterial; 0/DNA, Bacterial; 0/Epitopes; 0/RNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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