Document Detail


Genomic and functional analysis of the IncP-9 naphthalene-catabolic plasmid NAH7 and its transposon Tn4655 suggests catabolic gene spread by a tyrosine recombinase.
MedLine Citation:
PMID:  16707697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The naphthalene-catabolic (nah) genes on the incompatibility group P-9 (IncP-9) self-transmissible plasmid NAH7 from Pseudomonas putida G7 are some of the most extensively characterized genetic determinants for bacterial aerobic catabolism of aromatic hydrocarbons. In contrast to the detailed studies of its catabolic cascade and enzymatic functions, the biological characteristics of plasmid NAH7 have remained unclear. Our sequence determination in this study together with the previously deposited sequences revealed the entire structure of NAH7 (82,232 bp). Comparison of NAH7 with two other completely sequenced IncP-9 catabolic plasmids, pDTG1 and pWW0, revealed that the three plasmids share very high nucleotide similarities in a 39-kb region encoding the basic plasmid functions (the IncP-9 backbone). The backbone of NAH7 is phylogenetically more related to that of pDTG1 than that of pWW0. These three plasmids carry their catabolic gene clusters at different positions on the IncP-9 backbone. All of the NAH7-specified nah genes are located on a class II transposon, Tn4655. Our analysis of the Tn4655-encoded site-specific recombination system revealed that (i) a novel tyrosine recombinase, TnpI, catalyzed both the intra- and intermolecular recombination between two copies of the attI site, (ii) the functional attI site was located within a 119-bp segment, and (iii) the site-specific strand exchange occurred within a 30-bp segment in the 41-bp CORE site. Our results and the sequence data of other naphthalene-catabolic plasmids, pDTG1 and pND6-1, suggest a potential role of the TnpI-attI recombination system in the establishment of these catabolic plasmids.
Authors:
Masahiro Sota; Hirokazu Yano; Akira Ono; Ryo Miyazaki; Hidenori Ishii; Hiroyuki Genka; Eva M Top; Masataka Tsuda
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  188     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-18     Completed Date:  2006-07-25     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4057-67     Citation Subset:  IM    
Affiliation:
Department of Environmental Simulation, Institute for Environmental Sciences, Rokkasho, Aomori 039-3212, Japan. sota@uidaho.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB237655
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Base Sequence
DNA Transposable Elements*
Escherichia coli / genetics*
Escherichia coli Proteins / genetics,  metabolism
Gene Amplification
Genes, Bacterial
Molecular Sequence Data
Naphthalenes / metabolism*
Plasmids*
Polymerase Chain Reaction
Recombinases / genetics,  metabolism*
Restriction Mapping
Grant Support
ID/Acronym/Agency:
P20 RR 16448/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Transposable Elements; 0/Escherichia coli Proteins; 0/Naphthalenes; 0/Recombinases; 2166IN72UN/naphthalene
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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