Document Detail


Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames.
MedLine Citation:
PMID:  16690912     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genomic comparison of Neodiprion sertifer nucleopolyhedrovirus (NeseNPV) and Neodiprion lecontei nucleopolyhedrovirus (NeleNPV) showed that the hymenopteran baculoviruses had features in common and were distinct from other, fully sequenced lepidopteran and dipteran baculoviruses. Their genomes were small in size (86,462 and 81,755 bp, respectively), had low G+C contents (33.8 and 33.3 mol%, respectively) and contained fewer open reading frames (ORFs) (90 and 89, respectively) than other baculoviruses. They shared 69 ORFs (48.6% mean amino acid identity overall), 43 of which were previously identified baculovirus homologues. The remaining shared ORFs could be common to other baculoviruses, but low amino acid identities precluded identifying them as such. Some may also be unique to hymenopteran baculoviruses. These included a trypsin-like protease, a zinc-finger protein, regulator of chromosome condensation proteins, a densovirus capsid-like protein and a phosphotransferase. Structural analysis, the presence of conserved domains and phylogenetic studies suggested that some of these ORFs may be functional and could have been transferred horizontally from an insect host. ORFs found only in NeseNPV and NeleNPV may play a role in host specificity and/or tissue tropism, as hymenopteran baculoviruses are restricted to the midgut. The genomes were basically collinear, but contained non-syntenic regions (NSRs) with large numbers of repeats between their polyhedrin and dbp genes. They differed from each other in the number of ORFs and the G+C content of their NSRs and the presence of homologous regions in the NeseNPV genome. NeleNPV also had a short inversion relative to NeseNPV. NeseNPV contained 21 ORFs not found in NeleNPV and NeleNPV had 20 ORFs not found in NeseNPV.
Authors:
Hilary A M Lauzon; Alejandra Garcia-Maruniak; Paolo M de A Zanotto; José C Clemente; Elisabeth A Herniou; Christopher J Lucarotti; Basil M Arif; James E Maruniak
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  87     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-12     Completed Date:  2006-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1477-89     Citation Subset:  IM    
Affiliation:
Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Baculoviridae / genetics*
Genome, Viral*
Hymenoptera / virology*
Models, Molecular
Molecular Sequence Data
Nucleopolyhedrovirus / genetics*
Open Reading Frames / genetics*
Serine Endopeptidases / chemistry,  genetics
Viral Proteins / chemistry,  genetics
Chemical
Reg. No./Substance:
0/Viral Proteins; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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