Document Detail


Genomic anatomy of the hippocampus.
MedLine Citation:
PMID:  19109908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Availability of genome-scale in situ hybridization data allows systematic analysis of genetic neuroanatomical architecture. Within the hippocampus, electrophysiology and lesion and imaging studies demonstrate functional heterogeneity along the septotemporal axis, although precise underlying circuitry and molecular substrates remain uncharacterized. Application of unbiased statistical component analyses to genome-scale hippocampal gene expression data revealed robust septotemporal molecular heterogeneity, leading to the identification of a large cohort of genes with robust regionalized hippocampal expression. Manual mapping of heterogeneous CA3 pyramidal neuron expression patterns demonstrates an unexpectedly complex molecular parcellation into a relatively coherent set of nine expression domains in the septal/temporal and proximal/distal axes with reciprocal, nonoverlapping boundaries. Unique combinatorial profiles of adhesion molecules within these domains suggest corresponding differential connectivity, which is demonstrated for CA3 projections to the lateral septum using retrograde labeling. This complex, discrete molecular architecture provides a novel paradigm for predicting functional differentiation across the full septotemporal extent of the hippocampus.
Authors:
Carol L Thompson; Sayan D Pathak; Andreas Jeromin; Lydia L Ng; Cameron R MacPherson; Marty T Mortrud; Allison Cusick; Zackery L Riley; Susan M Sunkin; Amy Bernard; Ralph B Puchalski; Fred H Gage; Allan R Jones; Vladimir B Bajic; Michael J Hawrylycz; Ed S Lein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuron     Volume:  60     ISSN:  1097-4199     ISO Abbreviation:  Neuron     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-26     Completed Date:  2009-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1010-21     Citation Subset:  IM    
Affiliation:
Allen Institute for Brain Science, Seattle, WA 98103, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Brain Mapping*
Cholera Toxin / metabolism
Gene Expression Regulation, Developmental / physiology*
Genomics*
Hippocampus / anatomy & histology*,  physiology*
Imaging, Three-Dimensional
In Situ Hybridization / methods
Male
Mice
Mice, Inbred C57BL
Models, Biological
Nerve Tissue Proteins / genetics,  metabolism
Neural Cell Adhesion Molecules / genetics,  metabolism
Neural Pathways / anatomy & histology,  metabolism
Principal Component Analysis
Septum Pellucidum / anatomy & histology,  metabolism
Temporal Lobe / anatomy & histology,  metabolism
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Neural Cell Adhesion Molecules; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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