Document Detail


Genomic P elements content of a wild M' strain of Drosophila melanogaster: KP elements do not always function as type II repressor elements.
MedLine Citation:
PMID:  18379135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The P element is one of the best-studied DNA transposons as a model system to study evolution of mobile DNAs. The P element is a causative factor for P-M hybrid dysgenesis in Drosophila melanogaster and the P-M phenotype (P, Q, or M) has been thought to reflect genomic P elements content. Recent survey of natural populations showed that full-size P (FP) and KP elements are predominant in almost all current populations, irrespective of their phenotype variation. It was also suggested that some P elements are functionally inactive and their inactivation plays an important role in determining P-M phenotype. In order to know how the genomic P elements are inactivated, we characterized molecular features and insertion sites of them in an M' strain. We isolated 20 P elements, one FP, 15 KP, and four other internally deleted defective elements, all of which appeared thoroughly inactive. These FP and KP elements had canonical sequences in each case, but no mutations abolishing their function. In addition, they were mostly located in or within the vicinity of presumably active genes. Our results suggest that inactivation of P elements is associated with neither mutations nor constitutional suppression by heterochromatinization in M' strains and that only a few elements inserted in some special chromosomal regions are likely to be involved in determination of the phenotype of individual flies. Existence of many copies of canonical, but inactive, KP elements in the M' strain is inconsistent with the assumption that type II repression of the KP element is the main reason for its increase in the wild populations of D. melanogaster.
Authors:
Tomokazu Fukui; Yutaka Inoue; Masamitsu Yamaguchi; Masanobu Itoh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genes & genetic systems     Volume:  83     ISSN:  1341-7568     ISO Abbreviation:  Genes Genet. Syst.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-04-01     Completed Date:  2008-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607822     Medline TA:  Genes Genet Syst     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  67-75     Citation Subset:  IM    
Affiliation:
Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Sakyo, Kyoto, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA Transposable Elements*
Drosophila melanogaster / genetics*
Genes, Insect
Genome*
Mutation
Phenotype
Chemical
Reg. No./Substance:
0/DNA Transposable Elements

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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