Document Detail


Genomic DNA of leukemic patients: target for clinical diagnosis of MLL rearrangements.
MedLine Citation:
PMID:  16892314     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genomic DNA is the optimal resource to analyze questions concerning genetic changes that are related to oncogenesis. This article tries to summarize recent efforts to analyze chromosomal changes that trigger the development of human acute myeloid and lymphoblastic leukemias. The aim of this study was to establish an universal method that enables the identification and characterization of chromosomal translocations of the human MLL gene at the genomic nucleotide level. Chromosomal translocations of the MLL gene are the result of illegitimate recombination events in hematopoietic stem or precursor cells, strictly associated with the onset of highly malignant leukemic diseases. The applied technology was able to identify specific fusion alleles that were generated by chromosomal translocations, chromosomal deletions, chromosomal inversions and partial tandem duplications. Moreover, it allowed us to investigate even highly complex genetic changes by applying systematic breakpoint analyses. On the basis of these analyses, patient-specific molecular markers were established that turned out to be a very good source for monitoring minimal residual disease (MRD). MRD analyses control the efficiency and efficacy of current treatment protocols and have become a very sensitive molecular tool to monitor therapeutic success or failure in individual leukemia patients.
Authors:
Claus Meyer; Eric Kowarz; Björn Schneider; Clarissa Oehm; Thomas Klingebiel; Theo Dingermann; Rolf Marschalek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology journal     Volume:  1     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-08-07     Completed Date:  2006-12-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  656-63     Citation Subset:  IM    
Affiliation:
Institute of Pharmaceutical Biology, ZAFES, Diagnostic Center of Acute Leukemia, JWG-University of Frankfurt, Biocenter, Frankfurt/Main, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping
DNA Mutational Analysis
Genetic Markers / genetics
Genetic Predisposition to Disease / epidemiology,  genetics
Genetic Testing / methods*
Germany / epidemiology
Humans
Leukemia / diagnosis*,  epidemiology,  genetics*
Myeloid-Lymphoid Leukemia Protein / genetics*
Prevalence
Risk Assessment / methods*
Risk Factors
Chemical
Reg. No./Substance:
0/Genetic Markers; 0/MLL protein, human; 149025-06-9/Myeloid-Lymphoid Leukemia Protein

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