Document Detail


Genome-wide transcriptional profiling of mononuclear phagocytes recruited to mouse lungs in response to alveolar challenge with the TLR2 agonist Pam3CSK4.
MedLine Citation:
PMID:  19617307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Compared with the Toll-like receptor 4 (TLR4) ligand LPS restricted to gram-negative bacteria, few studies have addressed induction of lung inflammation and concomitant leukocyte recruitment in response to TLR2 ligands. This study is the first report showing that selective TLR2 stimulation by its ligand Pam(3)-Cys-Ser-Lys-Lys-Lys-Lys-OH (Pam(3)CSK(4)) within the alveolar compartment promoted lung inflammation in mice and induced the migration of circulatory immune cells including mononuclear phagocytes into the inflamed alveolar space. By using the transgenic CX(3)CR1(+/GFP) mouse strain for high-purity sorting of circulating and alveolar recruited mononuclear phagocytes together with SMART preamplification and whole genome oligonucleotide microarray techniques, we found that alveolar trafficking of mononuclear phagocytes was associated with profound changes of their gene expression profiles (approximately 900 differentially regulated genes postrecruitment). In particular, alveolar recruited mononuclear phagocytes showed upregulated transcripts of genes encoding cytokines/chemokines and pattern recognition receptor (PRR)-associated molecules. Notably, we observed a dynamic change of the genetic program of recruited mononuclear phagocytes obtained from bronchoalveolar lavage fluid at different time points (24 vs. 48 h) post-Pam(3)CSK(4) challenge. In early alveolar recruited mononuclear phagocytes, mRNA levels of both proinflammatory (e.g., TNF-alpha, CCL2, and IL-6) and central anti-inflammatory/ proresolution [e.g., IL-1-receptor antagonist (IL-1RN), CD200 receptor (CD200R), IL-1 receptor-associated kinase (IRAK-M), IL-10, and Bcl-2-associated X protein (Bax)] mediators were found to be highly upregulated simultaneously. In corresponding cells recruited until later time points, transcript levels of anti-inflammatory/proresolution molecules persisted at the same level, whereas mRNA levels of proinflammatory mediators were found to decline. Collectively, our in vivo study identifies genetic programs by which alveolar recruited mononuclear phagocytes may contribute to the development and termination of pneumonia caused by gram-positive bacteria.
Authors:
Maciej Cabanski; Jochen Wilhelm; Zbigniew Zaslona; Mirko Steinmüller; Ludger Fink; Werner Seeger; Jürgen Lohmeyer
Related Documents :
4319967 - Human alveolar macrophages: comparison of phagocytic ability, glucose utilization, and ...
10967407 - Dose and time dependent effects of caffeine on superoxide release, cell survival and dn...
20231397 - Azithromycin alters macrophage phenotype and pulmonary compartmentalization during lung...
12093687 - Epigallocatechin gallate, a potential immunomodulatory agent of tea components, diminis...
16876287 - Application and potential limitations of animal models utilized in the development of t...
23872327 - Illicium verum extract suppresses ifn-γ-induced icam-1 expression via blockade of jak/s...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-17
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  297     ISSN:  1522-1504     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L608-18     Citation Subset:  IM    
Affiliation:
Dept. of Internal Medicine, Division of Pulmonary and Critical Care Medicine and Infectious Diseases, Hannover Medical School, Carl-Neuberg-Str.1, 30625 Hannover, Germany. cabanski.maciej@mh-hannover.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bronchoalveolar Lavage Fluid / chemistry
Cell Movement
Cytokines / immunology,  metabolism
Flow Cytometry
Gene Expression Profiling*
Inflammation / etiology*,  pathology*
Lipopeptides / pharmacology
Lung / immunology*,  metabolism,  pathology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Oligonucleotide Array Sequence Analysis
Phagocytes / immunology*,  metabolism,  pathology
Pulmonary Alveoli / immunology*,  metabolism,  pathology
RNA, Messenger / genetics,  metabolism
Receptors, Chemokine / physiology
Reverse Transcriptase Polymerase Chain Reaction
Toll-Like Receptor 2 / agonists*,  metabolism
Chemical
Reg. No./Substance:
0/Cx3cr1 protein, mouse; 0/Cytokines; 0/Lipopeptides; 0/Pam(3)CSK(4) peptide; 0/RNA, Messenger; 0/Receptors, Chemokine; 0/Toll-Like Receptor 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Stability of irinotecan-loaded drug eluting beads (DC BeadTM) used for transarterial chemoembolisati...
Next Document:  The soluble guanylate cyclase activator HMR1766 reverses hypoxia-induced experimental pulmonary hype...