| Genome-wide transcriptional analysis of the human cell cycle identifies genes differentially regulated in normal and cancer cells. | |
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MedLine Citation:
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PMID: 18195366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Characterization of the transcriptional regulatory network of the normal cell cycle is essential for understanding the perturbations that lead to cancer. However, the complete set of cycling genes in primary cells has not yet been identified. Here, we report the results of genome-wide expression profiling experiments on synchronized primary human foreskin fibroblasts across the cell cycle. Using a combined experimental and computational approach to deconvolve measured expression values into "single-cell" expression profiles, we were able to overcome the limitations inherent in synchronizing nontransformed mammalian cells. This allowed us to identify 480 periodically expressed genes in primary human foreskin fibroblasts. Analysis of the reconstructed primary cell profiles and comparison with published expression datasets from synchronized transformed cells reveals a large number of genes that cycle exclusively in primary cells. This conclusion was supported by both bioinformatic analysis and experiments performed on other cell types. We suggest that this approach will help pinpoint genetic elements contributing to normal cell growth and cellular transformation. |
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Authors:
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Ziv Bar-Joseph; Zahava Siegfried; Michael Brandeis; Benedikt Brors; Yong Lu; Roland Eils; Brian D Dynlacht; Itamar Simon |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-01-14 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 105 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-24 Completed Date: 2008-02-12 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 955-60 Citation Subset: IM |
Affiliation:
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Department of Computer Science, School of Computer Science and Lane Center for Computational Biology, Carnegie Mellon University, Pittsburgh, PA 15213, USA. zivbj@cs.cmu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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genetics* Cell Cycle Proteins / classification, genetics* Cells, Cultured Computational Biology Flow Cytometry Gene Expression Profiling Gene Expression Regulation / genetics* Gene Expression Regulation, Neoplastic / genetics Genome, Human / genetics* Health* Humans Neoplasms / genetics*, pathology Transcription, Genetic / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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CA077245-11/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins |
| Comments/Corrections | |
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