Document Detail


Genome-wide transcription map of an archaeal cell cycle.
MedLine Citation:
PMID:  17307872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Relative RNA abundance was measured at different cell-cycle stages in synchronized cultures of the hyperthermophilic archaeon Sulfolobus acidocaldarius. Cyclic induction was observed for >160 genes, demonstrating central roles for transcriptional regulation and cell-cycle-specific gene expression in archaeal cell-cycle progression. Many replication genes were induced in a cell-cycle-specific manner, and novel replisome components are likely to be among the genes of unknown function with similar induction patterns. Candidate genes for the unknown genome segregation and cell division machineries were also identified, as well as seven transcription factors likely to be involved in cell-cycle control. Two serine-threonine protein kinases showed distinct cell-cycle-specific induction, suggesting regulation of the archaeal cell cycle also through protein modification. Two candidate recognition elements, CCR boxes, for transcription factors in control of cell-cycle regulons were identified among gene sets with similar induction kinetics. The results allow detailed characterization of the genome segregation, division, and replication processes and may, because of the extensive homologies between the archaeal and eukaryotic information machineries, also be applicable to core features of the eukaryotic cell cycle.
Authors:
Magnus Lundgren; Rolf Bernander
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-16
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  104     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-04-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2939-44     Citation Subset:  IM    
Affiliation:
Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, SE-752 36 Uppsala, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Archaea / cytology*,  genetics*
Cell Cycle / genetics*
Chromatin / genetics
DNA Replication / genetics
DNA, Archaeal
Databases, Genetic
Gene Expression Profiling
Gene Expression Regulation, Archaeal
Genes, Archaeal
Genome, Archaeal / genetics*
Polymerase Chain Reaction
Promoter Regions, Genetic / genetics
Regulatory Sequences, Nucleic Acid / genetics
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Chromatin; 0/DNA, Archaeal
Comments/Corrections

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