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Genome-wide microRNA and mRNA profiling in mouse liver development implicates mir302b and mir20a in repressing TGFβ signaling.
MedLine Citation:
PMID:  23315977     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
MicroRNAs (miRNAs) are recently discovered small RNA molecules that regulate developmental processes, such as proliferation, differentiation and apoptosis; however, the identity of miRNAs and their functions during liver development are largely unknown. Here, we investigated the miRNA and gene expression profiles for E8.5 endoderm, E14.5 Dlk1(+) liver cells (hepatoblasts), and adult liver by employing Illumina sequencing. We found that miRNAs were abundantly expressed at all three stages. Using K-means clustering analysis, thirteen miRNA clusters with distinct temporal expression patterns were identified. Mir302b, an endoderm enriched miRNA, was identified as a miRNA whose predicted targets are expressed highly in E14.5 hepatoblasts but low in the endoderm. We validated the expression of mir302b in the endoderm by whole-mount in situ hybridization. Interestingly, mir20a, the most highly expressed miRNA in the endoderm library, was also predicted to regulate some of the same targets as mir302b. We found that through targeting Tgfbr2, mir302b and mir20a are able to regulate TGFβ signal transduction. Moreover, mir302b can repress liver markers in an embryonic stem cell differentiation model. Collectively, we have uncovered dynamic patterns of individual miRNAs during liver development, as well as miRNA networks that could be essential for the specification and differentiation of liver progenitors. (HEPATOLOGY 2013.).
Authors:
Wei Wei; Juan Hou; Olivia Alder; Xin Ye; Sam Lee; Rebecca Cullum; Andy Chu; Yongjun Zhao; Stephanie M Warner; Darryl A Knight; Decheng Yang; Steven J M Jones; Marco A Marra; Pamela A Hoodless
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-12
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  -     ISSN:  1527-3350     ISO Abbreviation:  Hepatology     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 American Association for the Study of Liver Diseases.
Affiliation:
Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue Vancouver, B.C., V5Z1L3.
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