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Genome-wide enrichment screening reveals multiple targets and resistance genes for triclosan in Escherichia coli.
MedLine Citation:
PMID:  23124746     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Triclosan is a widely used biocide effective against different microorganisms. At bactericidal concentrations, triclosan appears to affect multiple targets, while at bacteriostatic concentrations, triclosan targets FabI. The site-specific antibiotic-like mode-of-action and a widespread use of triclosan in household products claimed to possibly induce cross-resistance to other antibiotics. Thus, we set out to define more systematically the genes conferring resistance to triclosan; A genomic library of Escherichia coli strain W3110 was constructed and enriched in a selective medium containing a lethal concentration of triclosan. The genes enabling growth in the presence of triclosan were identified by using a DNA microarray and confirmed consequently by ASKA clones overexpressing the selected 62 candidate genes. Among these, forty-seven genes were further confirmed to enhance the resistance to triclosan; these genes, including the FabI target, were involved in inner or outer membrane synthesis, cell-surface material synthesis, transcriptional activation, sugar phosphotransferase (PTS) systems, various transporter systems, cell division, and ATPase and reductase/dehydrogenase reactions. In particular, overexpression of pgsA, rcsA, or gapC conferred to E. coli cells a similar level of triclosan resistance induced by fabI overexpression. These results indicate that triclosan may have multiple targets other than well-known FabI and that there are several undefined novel mechanisms for the resistance development to triclosan, thus probably inducing cross antibiotic resistance.
Authors:
Byung Jo Yu; Jung Ae Kim; Hyun Mok Ju; Soo-Kyung Choi; Seung Jin Hwang; Sungyoo Park; Euijoong Kim; Jae-Gu Pan
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Publication Detail:
Type:  Journal Article     Date:  2012-11-04
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  50     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  785-91     Citation Subset:  IM    
Affiliation:
Systems and Synthetic Biology Research Center, Korea Research of Bioscience and Biotechnology (KRIBB), Daejeon, 305-701, Republic of Korea.
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