Document Detail


Genome-wide association analysis of Framingham Heart Study data for the Genetics Analysis Workshop 16: effects due to medication use.
MedLine Citation:
PMID:  20018045     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT : Problems associated with medication use and the consequent effects on genome-wide association analyses were explored using the Genetic Analysis Workshop 16 Problem 3 data. Lipid phenotypes were simulated in the Framingham Heart Study using several measured variables including causal genes (based on a 500 k SNP panel), smoking, dietary intake, and medication usage. We report a sensitivity analysis of how medication use (which artificially alters lipid values) affects association inferences. Associations were performed for LDL-c under seven different correction schemes: 1) ignore medication use entirely (no correction) and adjust for covariates; 2) delete medicated subjects then adjust for covariates; 3) include medication use (Yes/No) as a covariate during covariate adjustments; 4) correct raw values using clinical trials information then adjust for covariates; 5) correct raw values using the actual simulation protocol ("truth") then adjust for covariates; and 6-7) over-corrections (add arbitrary values) then adjust for covariates. Results indicate that failure to properly correct for medication usage can profoundly affect the heritability, and hence the association results. The empirical results yielded one genome-wide significant locus on chromosome 22 (RS2294207), consistent with the simulation protocol. This signal was detected under all schemes that corrected the raw values (clinical trials, simulation protocol, or over corrections), but was not detected under the first three adjustment schemes (ignore medication use, delete medicated individuals, use medication status as covariate). In summary, we confirm that failure to properly account for medication usage can have a profound impact on genetic associations.
Authors:
Treva K Rice; Yun Ju Sung; Gang Shi; C Charles Gu; Dc Rao
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Publication Detail:
Type:  Journal Article     Date:  2009-12-15
Journal Detail:
Title:  BMC proceedings     Volume:  3 Suppl 7     ISSN:  1753-6561     ISO Abbreviation:  BMC Proc     Publication Date:  2009  
Date Detail:
Created Date:  2009-12-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101316936     Medline TA:  BMC Proc     Country:  England    
Other Details:
Languages:  eng     Pagination:  S52     Citation Subset:  -    
Affiliation:
Division of Biostatistics, Washington University School of Medicine, Box 8067, 660 South Euclid Avenue, St Louis, Missouri 63110 USA. Treva@wubios.wustl.edu.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
R01 GM031575-28/GM/NIGMS NIH HHS
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