Document Detail


Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.
MedLine Citation:
PMID:  15788561     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The general transcriptional repressor Tup1 is responsible for the regulation of a large, diverse set of genes in Saccharomyces cerevisiae, and functional homologues of Tup1 have been identified in many metazoans. The crystal structure for the C-terminal portion of Tup1 has been solved and, when sequences of Tup1 homologues from fungi and metazoans were compared, a highly conserved surface was revealed. In this article, we analyze five point mutations that lie on this conserved surface. A statistical analysis of expression microarrays demonstrates that the mutant alleles are deficient in the repression of different subsets of Tup1-regulated genes. We were able to rank the mutant alleles of TUP1 based on the severity of their repression defects measured both by the number of genes derepressed and by the magnitude of that derepression. For one particular class of genes, the mutations on the conserved surface disrupted recruitment of Tup1 to the repressed promoters. However, for the majority of the genes derepressed by the Tup1 point mutants, recruitment of Tup1 to the regulated promoters is largely unaffected. These mutations affect the mechanism of repression subsequent to recruitment of the complex and likely represent a disruption of a mechanism that is conserved in fungi and metazoans. This work demonstrates that the evolutionarily conserved surface of Tup1 interacts with two separate types of proteins-sequence-specific DNA-binding proteins responsible for recruiting Tup1 to promoters as well as components that are likely to function in a conserved repression mechanism.
Authors:
Sarah R Green; Alexander D Johnson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2005-03-23
Journal Detail:
Title:  Molecular biology of the cell     Volume:  16     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-03     Completed Date:  2005-11-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2605-13     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of California-San Francisco, San Francisco, CA 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Alanine / metabolism
Alleles
Amino Acid Substitution
Chromatin Immunoprecipitation
DNA-Binding Proteins / chemistry,  genetics,  metabolism*
Fungal Proteins / chemistry,  genetics,  metabolism*
Gene Expression Regulation, Fungal
Genes, Fungal
Genome, Fungal*
Models, Biological
Models, Molecular
Nuclear Proteins / chemistry,  genetics,  metabolism*
Oligonucleotide Array Sequence Analysis
Point Mutation
Promoter Regions, Genetic
Protein Structure, Tertiary
Repressor Proteins / chemistry,  genetics,  metabolism*
Saccharomyces cerevisiae / genetics,  metabolism
Saccharomyces cerevisiae Proteins / chemistry,  genetics,  metabolism*
Transcription, Genetic
beta-Galactosidase / analysis,  metabolism
Grant Support
ID/Acronym/Agency:
GM-37049/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Fungal Proteins; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; 0/TUP1 protein, S cerevisiae; 56-41-7/Alanine; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

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