| Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs). | |
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MedLine Citation:
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PMID: 18353984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Small RNAs (21-24 nt) are involved in gene regulation through translation inhibition, mRNA cleavage, or directing chromatin modifications. In rice, currently approximately 240 microRNAs (miRNAs) have been annotated. We sequenced more than four million small RNAs from rice and identified another 24 miRNA genes. Among these, we found a unique class of miRNAs that derive from natural cis-antisense transcript pairs. This configuration generates miRNAs that can perfectly match their targets. We provide evidence that the miRNAs function by inducing mRNA cleavage in the middle of their complementary site. Their production requires Dicer-like 1 (DCL1) activity, which is essential for canonical miRNA biogenesis. All of the natural antisense miRNAs (nat-miRNAs) identified in this study have large introns in their precursors that appear critical for nat-miRNA evolution and for the formation of functional miRNA loci. These findings suggest that other natural cis-antisense loci with similar exon-intron arrangements could be another source of miRNA genes. |
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Authors:
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Cheng Lu; Dong-Hoon Jeong; Karthik Kulkarni; Manoj Pillay; Kan Nobuta; Rana German; Shawn R Thatcher; Christopher Maher; Lifang Zhang; Doreen Ware; Bin Liu; Xiaofeng Cao; Blake C Meyers; Pamela J Green |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-03-19 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 105 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-26 Completed Date: 2008-04-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 4951-6 Citation Subset: IM |
Affiliation:
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Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE10523 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Conserved Sequence Gene Expression Regulation, Plant Gene Library Genome, Plant / genetics* MicroRNAs / biosynthesis, chemistry, genetics* Models, Biological Molecular Sequence Data Nucleic Acid Conformation Oryza sativa / genetics* Plant Proteins / genetics, metabolism RNA, Antisense / genetics* RNA, Messenger / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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T32 GM-08550/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs; 0/Plant Proteins; 0/RNA, Antisense; 0/RNA, Messenger |
| Comments/Corrections | |
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