Document Detail


Genome-wide analyses reveal properties of redundant and specific promoter occupancy within the ETS gene family.
MedLine Citation:
PMID:  17652178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The conservation of in vitro DNA-binding properties within families of transcription factors presents a challenge for achieving in vivo specificity. To uncover the mechanisms regulating specificity within the ETS gene family, we have used chromatin immunoprecipitation coupled with genome-wide promoter microarrays to query the occupancy of three ETS proteins in a human T-cell line. Unexpectedly, redundant occupancy was frequently detected, while specific occupancy was less likely. Redundant binding correlated with housekeeping classes of genes, whereas specific binding examples represented more specialized genes. Bioinformatics approaches demonstrated that redundant binding correlated with consensus ETS-binding sequences near transcription start sites. In contrast, specific binding sites diverged dramatically from the consensus and were found further from transcription start sites. One route to specificity was found--a highly divergent binding site that facilitates ETS1 and RUNX1 cooperative DNA binding. The specific and redundant DNA-binding modes suggest two distinct roles for members of the ETS transcription factor family.
Authors:
Peter C Hollenhorst; Atul A Shah; Christopher Hopkins; Barbara J Graves
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-07-24
Journal Detail:
Title:  Genes & development     Volume:  21     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-02     Completed Date:  2007-09-26     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1882-94     Citation Subset:  IM    
Affiliation:
Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites / genetics
Cell Line
Consensus Sequence
Core Binding Factor Alpha 2 Subunit / genetics,  metabolism
DNA / genetics,  metabolism
Genome, Human
Humans
Jurkat Cells
Multigene Family*
Phylogeny
Promoter Regions, Genetic*
Proto-Oncogene Protein c-ets-1 / genetics,  metabolism
Proto-Oncogene Proteins c-ets / genetics*,  metabolism
Proto-Oncogenes*
T-Lymphocytes / metabolism
Grant Support
ID/Acronym/Agency:
P01 CA24014/CA/NCI NIH HHS; R01 GM38663/GM/NIGMS NIH HHS; T32 CA93247/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Core Binding Factor Alpha 2 Subunit; 0/ETS1 protein, human; 0/Proto-Oncogene Protein c-ets-1; 0/Proto-Oncogene Proteins c-ets; 0/RUNX1 protein, human; 9007-49-2/DNA
Comments/Corrections

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