| Genome segment 6 of Antheraea mylitta cypovirus encodes a structural protein with ATPase activity. | |
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MedLine Citation:
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PMID: 18486179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The genome segment 6 (S6) of the 11 double stranded RNA genomes from Antheraea mylitta cypovirus was converted into cDNA, cloned and sequenced. S6 consisted of 1944 nucleotides with an ORF of 607 amino acids and could encode a protein of 68 kDa, termed P68. Motif scan and molecular docking analysis of P68 showed the presence of two cystathionine beta synthase (CBS) domains and ATP binding sites. The ORF of AmCPV S6 was expressed in E. coli as His-tag fusion protein and polyclonal antibody was raised. Immunoblot analysis of virus infected gut cells and purified polyhedra using raised anti-p68 polyclonal antibody showed that S6 encodes a viral structural protein. Fluorescence and ATPase assay of soluble P68 produced in Sf-9 cells via baculovirus expression system showed its ability to bind and cleave ATP. These results suggest that P68 may bind viral RNA through CBS domains and help in replication and transcription through ATP binding and hydrolysis. |
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Authors:
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Venkata R M Chavali; Chaithanya Madhurantakam; Suvankar Ghorai; Sobhan Roy; Amit K Das; Ananta K Ghosh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-16 |
Journal Detail:
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Title: Virology Volume: 377 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-16 Completed Date: 2008-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 7-18 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Indian Institute of Technology, Kharagpur, Kharagpur 721302, West Bengal, India. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EF062995 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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chemistry,
genetics*,
metabolism Amino Acid Sequence Animals Base Sequence Catalytic Domain Cloning, Molecular DNA, Viral / genetics Genome, Viral Models, Molecular Molecular Sequence Data Moths / virology* Phylogeny Protein Conformation Protein Structure, Secondary Recombinant Fusion Proteins / chemistry, genetics, metabolism Reoviridae / enzymology*, genetics* Sequence Homology, Amino Acid Viral Structural Proteins / chemistry, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Recombinant Fusion Proteins; 0/Viral Structural Proteins; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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