| Genome-scale identification of UDP-GlcNAc-dependent pathways. | |
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MedLine Citation:
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PMID: 18646010 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Metabolite flux to UDP-GlcNAc and Golgi N-glycan biosynthesis regulates surface residency of glycoprotein receptors and transporters, and thus sensitivities of cells to extracellular cues. Salvage of GlcNAc increases UDP-GlcNAc and branching of N-glycans progressively, but displays an optimum for cell proliferation and bulk endocytosis in mouse NMuMG and human HEK293T epithelial cells. In this report, we measured global changes in gene expression in low and high GlcNAc-supplemented cells. Genes upregulated by high GlcNAc included the EGF and TGF-beta signaling pathways and cell cycle checkpoint, while downregulated genes indicated lower metabolic activity. Genes increased or decreased by high GlcNAc were assessed by transfecting cells with small interfering RNA (siRNA) and measuring effects on three phenotypes: proliferation and bulk endocytosis, and beta1,6GlcNAc-branching of N-glycans. siRNA targeting LGALS3, WBSCR17, PHF3, SDC2 and CTNNAL1 partially reversed the GlcNAc-induced phenotypes, suggesting a role for galectin-3/N-glycans, proteoglycans, O-glycans, and junctional cell adhesion. |
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Authors:
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Ken S Lau; Sofia Khan; James W Dennis |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 8 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-13 Completed Date: 2008-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 3294-302 Citation Subset: IM |
Affiliation:
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Samuel Lunenfeld Research Institute, Mount Sinai Hospital, ON, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Proliferation / drug effects Chromatography, High Pressure Liquid Endocytosis / drug effects Gene Expression Profiling* Genome, Human / genetics* Humans Oligonucleotide Array Sequence Analysis RNA, Small Interfering / genetics Signal Transduction / drug effects, physiology* Uridine Diphosphate N-Acetylglucosamine / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 528-04-1/Uridine Diphosphate N-Acetylglucosamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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