Document Detail


Genome organization of major tandem repeats in the hard tick, Ixodes scapularis.
MedLine Citation:
PMID:  20306126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In spite of the global medical and veterinary importance of Ixodid ticks, relatively little is known about their genome organization. To address this, we developed the first fluorescence in situ hybridization (FISH)-based chromosome markers in the Lyme disease vector, Ixodes scapularis. Shotgun genomic DNA (gDNA) sequences were used to identify three major tandem repeat families which were localized to specific heterochromatic regions of I. scapularis chromosomes prepared from the mitotic cell line ISE18. Together, these repeats were estimated to contribute approximately 159 Mb (8%) of the 2.1 Gb (haploid) I. scapularis genome. The relative arrangement of each tandem repeat family and the nucleolar organizing regions was determined by rehybridization to individual chromosome spreads, which was useful to distinguish different chromosomes in the ISE18 karyotype. Long stretches (>20 kb) of tandem repeat-containing gDNA were resistant to digestion by the methylation-sensitive restriction enzyme HpaII and localized to the presumed peri-centromeric regions of the chromosomes. A telomeric probe based on the arthropod-conserved (TTAGG)(n) tandemly repetitive motif was localized to the termini of each I. scapularis chromosome. Localization of these markers produced the first link between DNA sequences and major structural features of I. scapularis chromosomes and thereby provided the framework for a FISH-based physical map.
Authors:
Jason M Meyer; Timothy J Kurtti; Janice P Van Zee; Catherine A Hill
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-20
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  18     ISSN:  1573-6849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  357-70     Citation Subset:  IM    
Affiliation:
Department of Entomology, Purdue University, West Lafayette, IN 47907-2089, USA. meyerjm@purdue.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Centromere / genetics
Computational Biology
Consensus Sequence
Cytogenetic Analysis
DNA / genetics
DNA Probes / metabolism
Genome / genetics*
In Situ Hybridization, Fluorescence
Ixodes / genetics*
Molecular Sequence Data
Software
Tandem Repeat Sequences / genetics*
Telomere / genetics
Grant Support
ID/Acronym/Agency:
HHS2662004000396//PHS HHS
Chemical
Reg. No./Substance:
0/DNA Probes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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