Document Detail

Genome expansion and differential expression of amino acid transporters at the aphid/Buchnera symbiotic interface.
MedLine Citation:
PMID:  21613235     Owner:  NLM     Status:  Publisher    
In insects some of the most ecologically important symbioses are nutritional symbioses that provide hosts with novel traits and thereby facilitate exploitation of otherwise inaccessible niches. One such symbiosis is the ancient obligate intracellular symbiosis of aphids with the γ-proteobacteria, Buchnera aphidicola. While the nutritional basis of the aphid/Buchnera symbiosis is well understood, the processes and structures that mediate the intimate interactions of symbiotic partners remain uncharacterized. Here, using a de novo approach we characterize the complement of 40 amino acid polyamine organocation (APC) superfamily member amino acid transporters encoded in the genome of the pea aphid, Acyrthosiphum pisum. We find that the A. pisum APC superfamily is characterized by extensive gene duplications such that A. pisum has more APC superfamily transporters than other fully sequenced insects, including a 10 paralog aphid-specific expansion of the APC transporter slimfast. Detailed expression analysis of 17 transporters selected on the basis of their phylogenetic relationship to five amino acid transporters identified in an earlier bacteriocyte EST study distinguished a subset of eight transporters that have been recruited for amino acid transport in bacteriocyte cells at the symbiotic interface. These eight transporters include transporters that are highly expressed and/or highly enriched in bacteriocytes and intriguingly, the four amino acid transporters that show bacteriocyte-enriched expression are all members of gene family expansions, while three of the four that are highly expressed but not enriched in bacteriocytes retain one-to-one orthology with transporters in other genomes. Finally, analysis of evolutionary rates within the large A. pisum slimfast expansion demonstrated increased rates of molecular evolution coinciding with two major shifts in expression: (1) a loss of gut expression and possibly a gain of bacteriocyte expression, and (2) loss of expression in all surveyed tissues in asexual females. Taken together, our characterization of nutrient amino acid transporters at the aphid/Buchnera symbiotic interface provides the first examination of the processes and structures operating at the interface of an obligate intracellular insect nutritional symbiosis, offering unique insight into the types of genomic change that likely facilitated evolutionary maintenance of the symbiosis.
Daniel R G Price; Rebecca P Duncan; Shuji Shigenobu; Alex C C Wilson
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-24
Journal Detail:
Title:  Molecular biology and evolution     Volume:  -     ISSN:  1537-1719     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8501455     Medline TA:  Mol Biol Evol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biology, University of Miami, Coral Gables, FL 33146, USA.
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