Document Detail


Genome analysis with gene expression microarrays.
MedLine Citation:
PMID:  8639166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Advances in biochemistry, chemistry and engineering have enabled the development of a new gene expression assay. This 'chip-based' approach utilizes microscopic arrays of cDNAs printed on glass as high-density hybridization targets. Fluorescent probe mixtures derived from total cellular messenger RNA (mRNA) hybridize to cognate elements on the array, allowing accurate measurement of the expression of the corresponding genes. Array densities of > 1,000 cDNAs per cm2 enable quantitative expression monitoring of a large number of genes in a single hybridization. A two-color fluorescence detection scheme allows rapid and simultaneous differential expression analysis of independent biological samples. Mass-produced microarrays provide a new tool for genome expression analysis that may revolutionize genetic dissection, drug discovery and human disease diagnostics.
Authors:
M Schena
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  BioEssays : news and reviews in molecular, cellular and developmental biology     Volume:  18     ISSN:  0265-9247     ISO Abbreviation:  Bioessays     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-10     Completed Date:  1996-07-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510851     Medline TA:  Bioessays     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  427-31     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305-5307, USA. schena@cmgm.stanford.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Complementary
Gene Expression*
Genetic Techniques*
Genome*
Genome, Human
Humans
Polymerase Chain Reaction / methods
RNA, Messenger / analysis
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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