Document Detail


Genome and transcriptome profiles of CD133-positive colorectal cancer cells.
MedLine Citation:
PMID:  21435437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Colorectal carcinomas (CRC) might be organized hierarchically and contain a subpopulation of tumorigenic, putative cancer stem cells that are CD133 positive. We studied the biological and genetic characteristics of such cells in CRC cell lines and primary tumors. Three CRC cell lines were sorted in CD133 positive and negative fractions. The respective genetic aberration profiles were studied using array comparative genomic hybridization (aCGH) and expression profiling. Tumorigenicity for each cellular population was tested by injection into nude mice. Additionally, we compared CD133+ and CD133- cells of 12 primary colorectal tumors using laser capture microdissection and aCGH. Three of five CRC cell lines displayed both CD133+ and CD133- cells, but tumorigenicity of these subfractions did not differ significantly and aCGH revealed essentially identical genomic imbalances. However, 96 genes were differentially expressed between the two populations. Array comparative genomic hybridization analysis after laser capture microdissection of CD133+ and CD133- areas in primary colorectal tumors revealed genetic differences in 7 of 12 cases. The use of cell lines for studying genomic alterations that define cancer stem cell characteristics, therefore, seems questionable. In contrast, CD133+ cells in primary cancer samples showed a unique genomic aberration profile. In conclusion, our data suggest that CD133 positivity defines a genetically distinct cellular compartment in primary CRC, which potentially includes tumor initiating cells.
Authors:
Timo Gaiser; Jordi Camps; Sandra Meinhardt; Danny Wangsa; Quang Tri Nguyen; Sudhir Varma; Claudia Dittfeld; Leoni A Kunz-Schughart; Ralf Kemmerling; Maria R Becker; Kerstin Heselmeyer-Haddad; Thomas Ried
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of pathology     Volume:  178     ISSN:  1525-2191     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-25     Completed Date:  2011-07-15     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1478-88     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
Affiliation:
Section of Cancer Genomics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / biosynthesis*
Biopsy / methods
Caco-2 Cells
Cell Line, Tumor
Chromosome Aberrations
Colorectal Neoplasms / metabolism*
Comparative Genomic Hybridization
Flow Cytometry / methods
Genome
Genomics / methods
Glycoproteins / biosynthesis*
Humans
Lasers
Mice
Mice, Nude
Microdissection
Neoplasm Transplantation
Peptides
Transcription, Genetic
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides
Comments/Corrections

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