Document Detail


Genital blood flow and endometrial gene expression during the preovulatory period after Prostaglandin F(2alpha)-induced luteolysis in different luteal phases in cows.
MedLine Citation:
PMID:  19305127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to examine the hypothesis that follicular and uterine perfusion as well as endometrial gene expression during the ovulatory period differs after induction of luteolysis during the 1(st) follicular wave compared with the 2(nd) wave or in intact cycle. Nine healthy non-lactating Holstein-Friesian cows were examined during three estrous cycles. A prostaglandin F(2alpha) analogue (PGF) was administered randomly either on Day 7 (1(st) wave cycle) or Day 11 (2(nd) wave cycle) after detection of ovulation (=Day 1). No hormonal treatment was used in the intact cycle with spontaneous ovulation. Transrectal Doppler sonography was conducted daily after PGF injection and in the intact cycle beginning on Day 18 of the estrous cycle until ovulation. Follicular blood flow (FBF) was determined by measuring the maximum area of colour pixels on digitalized images of the follicles. Uterine blood flow was quantified by the time-averaged maximum velocity (UTAMV) and pulsatility index (PI) in both uterine arteries. Blood flow measurements were carried out on Days -1 and 0. Endometrial biopsy specimens were taken on Day 1 and analyzed for the gene expressions of estrogen, progesterone, oxytocin and VEGF receptors and eNOS and iNOS using RT-PCR. The interval from PGF injection to ovulation was shorter (P<0.05) in 1(st) wave cycles than in 2(nd) wave cycles. On Days 0 and -1, FBF was greater (P<0.05) in 1(st) wave cycles than in intact and 2(nd) wave cycles. On Day -1, UTAMV was greater (P<0.05) in 1(st) wave cycles than in intact and 2(nd) wave cycles. There were no differences (P>0.05) in FBF and UTAMV values between 2(nd) wave and intact cycles. No differences (P>0.05) were detected in the gene expressions of endometrial receptors and enzymes between intact, 1(st) and 2(nd) wave cycles. The results show that follicular and uterine perfusion during the ovulatory phase are higher after induction of luteolysis during the 1(st) follicular wave compared with the 2(nd) wave or intact cycle. There were no effects on endometrial gene expression.
Authors:
Anne Jordan; Kathrin Herzog; Susanne E Ulbrich; Nicola Beindorff; Anne Honnens; Lars Krüger; Akio Miyamoto; Heinrich Bollwein
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial     Date:  2009-03-19
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  55     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-07-02     Completed Date:  2009-08-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  309-15     Citation Subset:  IM    
Affiliation:
Clinic for Cattle, University of Veterinary Medicine Hannover.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Dinoprost / pharmacology*
Endometrium / metabolism*
Estradiol / blood
Female
Gene Expression / physiology
Genitalia, Female / blood supply*,  metabolism
Luteal Phase / blood,  drug effects*,  genetics
Luteolysis / blood,  drug effects*,  genetics
Ovarian Follicle / drug effects,  metabolism
Ovulation / blood,  drug effects,  genetics*,  physiology
Progesterone / blood
Regional Blood Flow / physiology
Chemical
Reg. No./Substance:
50-28-2/Estradiol; 551-11-1/Dinoprost; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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