Document Detail


Geniposide inhibits CoCl2-induced PC12 cells death via the mitochondrial pathway.
MedLine Citation:
PMID:  20092796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A number of studies have shown that oxidative stress and mitochondrial involvement are major triggering factors in the development of neurodegenerative diseases. Cobalt chloride (CoCl(2))-induced cell death in PC12 cells may serve a simple and convenient in vitro model of hypoxia-induced neuronal cytotoxicity. To explore the effect of geniposide on CoCl(2) which induced cytotoxicity and mitochondrial function in rat pheochromocytoma PC12 cells, we analyzed the influence of geniposide on the expression of apoptosis-related proteins.
METHODS: PC12 cells and RNAi PC12 cells were treated with 0, 12.5, 25, 50, 100 micromol/L geniposide for 12 hours and then exposure to 400 micromol/L CoCl(2) for 12 hours. Cell viability, cell morphology, and expression of Bcl-2, Bax, P53 and caspase-9 were determined using Western blotting.
RESULTS: Pretreatment with geniposide markedly improved the cells viability and morphology, decreased the expression of Bax, P53 and caspase-9, and increased the expression of Bcl-2 in PC12 cells challenged by CoCl(2)2. However, in the RNAi PC12 cells, geniposide had no significant effect on the expression of these proteins.
CONCLUSION: Geniposide protects PC12 cells from CoCl(2) involved in mitochondrial mediated apoptosis, and GLP-1R might play a critical role in the neuroprotection of geniposide in PC12 cells.
Authors:
Li-xia Guo; Jian-hui Liu; Zhi-ning Xia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chinese medical journal     Volume:  122     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-22     Completed Date:  2010-03-30     Revised Date:  2013-03-19    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  2886-92     Citation Subset:  IM    
Affiliation:
Research Center of Pharmaceutical Chemistry & Chemical Biology, Chongqing Technology and Business University, Chongqing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Cobalt / toxicity*
Iridoids / pharmacology*
Mitochondria / physiology*
Neuroprotective Agents / pharmacology*
PC12 Cells
Proto-Oncogene Proteins c-bcl-2 / physiology
Rats
Receptors, Glucagon / drug effects,  physiology
Signal Transduction
bcl-2-Associated X Protein / physiology
Chemical
Reg. No./Substance:
0/Bax protein, rat; 0/Iridoids; 0/Neuroprotective Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Glucagon; 0/bcl-2-Associated X Protein; 0/glucagon-like peptide-1 receptor; 24512-63-8/geniposide; 7440-48-4/Cobalt; 7646-79-9/cobaltous chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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