Document Detail


Genetically modified organisms in food-screening and specific detection by polymerase chain reaction.
MedLine Citation:
PMID:  10606569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PCR methods for the detection of genetically modified organisms (GMOs) were developed that can be used for screening purposes and for specific detection of glyphosate-tolerant soybean and insect-resistant maize in food. Primers were designed to amplify parts of the 35S promoter derived from Cauliflower Mosaic Virus, the NOS terminator derived from Agrobacterium tumefaciens and the antibiotic marker gene NPTII (neomycin-phosphotransferase II), to allow for general screening of foods. PCR/hybridization protocols were established for the detection of glyphosate-tolerant RoundUp Ready soybean and insect-resistant Bt-maize. Besides hybridization, confirmation of the results using restriction analysis was also possible. The described methods enabled a highly sensitive and specific detection of GMOs and thus provide a useful tool for routine analysis of raw and processed food products.
Authors:
S Vollenhofer; K Burg; J Schmidt; H Kroath
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  47     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-04-11     Completed Date:  2000-04-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5038-43     Citation Subset:  IM    
Affiliation:
Austrian Research Centers Seibersdorf, Biotechnology Unit, A-2444 Seibersdorf, Austria. sabine.vollenhofer@arcs.ac.at
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MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
DNA Primers
DNA, Plant / analysis
Genetic Engineering
Humans
Nucleic Acid Hybridization
Polymerase Chain Reaction
Soybeans / genetics*
Zea mays / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Plant
Comments/Corrections
Erratum In:
J Agric Food Chem 2001 Jul;49(7):3508

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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