Document Detail

Genetically modified fibroblasts producing NGF protect hippocampal neurons after ischemia in the rat.
MedLine Citation:
PMID:  7605924     Owner:  NLM     Status:  MEDLINE    
The neuroprotective effect of nerve growth factor (NGF) on the pyramidal cells in the vulnerable CA1-CA2 sectors of the hippocampus was investigated in a rat model of transient forebrain ischemia. A genetically modified fibroblast line that secretes high levels of NGF was implanted 7 days before induction of ischemia between the hippocampal CA1-CA2 subfields in the right hemisphere. Rats were then subjected to 10 min of cerebral ischemia in a four vessel occlusion model. Morphological changes in the CA1 and CA2 subfields were evaluated 7 days after ischemia. Animals in the NGF-protected group had significantly higher numbers of normal appearing neurons in the right CA1 and CA2 regions, compared with their non-implanted left hemispheres, to non-implanted animals or to animals implanted with non-modified cells. The data confirmed that NGF can protect CA1-CA2 hippocampal neurons from ischemic damage by implantation of genetically engineered cells producing NGF.
P A Pechan; T Yoshida; N Panahian; M A Moskowitz; X O Breakefield
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  6     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-08-17     Completed Date:  1995-08-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  669-72     Citation Subset:  IM    
Molecular Neurogenetics Unit, Massachusetts General Hospital East, Harvard Medical School, Charlestown 02129, USA.
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MeSH Terms
Cell Line
Fibroblasts / metabolism*,  transplantation
Gene Therapy*
Hippocampus / cytology,  drug effects
Ischemic Attack, Transient / drug therapy*
Nerve Growth Factors / pharmacology*
Neurons / drug effects
Prosencephalon / blood supply*
Rats, Wistar
Grant Support
Reg. No./Substance:
0/Nerve Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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