Document Detail


Genetically modified fibroblasts producing NGF protect hippocampal neurons after ischemia in the rat.
MedLine Citation:
PMID:  7605924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neuroprotective effect of nerve growth factor (NGF) on the pyramidal cells in the vulnerable CA1-CA2 sectors of the hippocampus was investigated in a rat model of transient forebrain ischemia. A genetically modified fibroblast line that secretes high levels of NGF was implanted 7 days before induction of ischemia between the hippocampal CA1-CA2 subfields in the right hemisphere. Rats were then subjected to 10 min of cerebral ischemia in a four vessel occlusion model. Morphological changes in the CA1 and CA2 subfields were evaluated 7 days after ischemia. Animals in the NGF-protected group had significantly higher numbers of normal appearing neurons in the right CA1 and CA2 regions, compared with their non-implanted left hemispheres, to non-implanted animals or to animals implanted with non-modified cells. The data confirmed that NGF can protect CA1-CA2 hippocampal neurons from ischemic damage by implantation of genetically engineered cells producing NGF.
Authors:
P A Pechan; T Yoshida; N Panahian; M A Moskowitz; X O Breakefield
Related Documents :
24567464 - Three-dimensional endovaginal sonography of synthetic implanted materials in the female...
23664574 - Primary breast augmentation clinical trial outcomes stratified by surgical incision, an...
25074974 - Standards for device implantation and follow-up: personnel, equipment, and facilities: ...
22734654 - Deficiencies in patients' comprehension of implantable cardioverter defibrillator therapy.
14750004 - Measuring and modelling plant area index in beech stands.
10872964 - Effects of diode and nd:yag laser irradiation on titanium discs: a scanning electron mi...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  6     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-08-17     Completed Date:  1995-08-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  669-72     Citation Subset:  IM    
Affiliation:
Molecular Neurogenetics Unit, Massachusetts General Hospital East, Harvard Medical School, Charlestown 02129, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Fibroblasts / metabolism*,  transplantation
Gene Therapy*
Hippocampus / cytology,  drug effects
Ischemic Attack, Transient / drug therapy*
Male
Nerve Growth Factors / pharmacology*
Neurons / drug effects
Prosencephalon / blood supply*
Rats
Rats, Wistar
Grant Support
ID/Acronym/Agency:
NS10828/NS/NINDS NIH HHS; NS24279/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Activation of tyrosine hydroxylase by haloperidol in fetal nigral transplants.
Next Document:  Fragmentation of the neuronal cytoskeleton in the Lewy body variant of Alzheimer's disease.