Document Detail


Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods.
MedLine Citation:
PMID:  8597157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
Authors:
L H Thompson; R W Wu; J S Felton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicology letters     Volume:  82-83     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-04-15     Completed Date:  1996-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  883-9     Citation Subset:  IM    
Affiliation:
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
CHO Cells
Cricetinae
Cytochrome P-450 Enzyme System / biosynthesis
Imidazoles / metabolism,  toxicity*
Mutagens / toxicity*
Mutation
Quinolines / toxicity*
Grant Support
ID/Acronym/Agency:
CA55861/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Imidazoles; 0/Mutagens; 0/Quinolines; 105650-23-5/2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; 76180-96-6/2-amino-3-methylimidazo(4,5-f)quinoline; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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