| Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods. | |
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MedLine Citation:
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PMID: 8597157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines. |
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Authors:
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L H Thompson; R W Wu; J S Felton |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Toxicology letters Volume: 82-83 ISSN: 0378-4274 ISO Abbreviation: Toxicol. Lett. Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1996-04-15 Completed Date: 1996-04-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 883-9 Citation Subset: IM |
Affiliation:
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Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biotransformation CHO Cells Cricetinae Cytochrome P-450 Enzyme System / biosynthesis Imidazoles / metabolism, toxicity* Mutagens / toxicity* Mutation Quinolines / toxicity* |
| Grant Support | |
ID/Acronym/Agency:
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CA55861/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Imidazoles; 0/Mutagens; 0/Quinolines; 105650-23-5/2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; 76180-96-6/2-amino-3-methylimidazo(4,5-f)quinoline; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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