Document Detail


Genetically engineering mammalian cell lines for increased viability and productivity.
MedLine Citation:
PMID:  14545894     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The generation of new host cell lines for the production of foreign proteins can be achieved by cell engineering. This approach can be used to enhance the cell's ability to produce proteins that are properly processed and secreted at elevated levels and consequently can increase the overall productivity of an expression system. One potential target for cell engineering is the modification of the cell's protein folding capacity. The appropriate folding, assembly, localization and secretion of newly synthesized proteins is dependent upon the action of a group of proteins known as molecular chaperones. Improving the host cell's chaperoning capacity might increase the yield of properly folded recombinant proteins by preventing the formation of insoluble aggregates. Another potentially beneficial cell engineering goal is the inhibition of physiological cell death. The productivity of genetically engineered cells is dependent upon the maintenance of high levels of cell viability throughout the bioprocess period. Fluctuations in a cell's environment can trigger a deliberate form of cell death known as apoptosis. The proteins that mediate this self-destruction are currently being characterized. Regulating the expression of these death genes by cellular engineering could limit the loss of productivity that results from the physiological death of the recombinant cell line.
Authors:
D D Mosser; B Massie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology advances     Volume:  12     ISSN:  0734-9750     ISO Abbreviation:  Biotechnol. Adv.     Publication Date:  1994  
Date Detail:
Created Date:  2003-10-10     Completed Date:  2003-12-19     Revised Date:  2006-07-07    
Medline Journal Info:
Nlm Unique ID:  8403708     Medline TA:  Biotechnol Adv     Country:  England    
Other Details:
Languages:  eng     Pagination:  253-77     Citation Subset:  -    
Affiliation:
Biotechnology Research Institute, National Research Council of Canada, Montréal, Québec, Canada.
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